The Effect of BSCL2 Gene on Fat Deposition Traits in Pigs
Author:
Piórkowska Katarzyna1ORCID, Sroka Julia2, Żukowski Kacper3ORCID, Zygmunt Karolina1ORCID, Ropka-Molik Katarzyna1ORCID, Tyra Mirosław4ORCID
Affiliation:
1. Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland 2. Department of Biotechnology and Horticulture, University of Agricultural in Kraków, 29-go Listopada 54, 31-425 Kraków, Poland 3. Department of Cattle Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland 4. Department of Pig Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
Abstract
BSCL2 encodes seipin, a transmembrane endoplasmic reticulum protein associated with lipodystrophy and severe metabolic complications, including diabetes and hepatic steatosis. In pigs, BSCL2 expression increases during adipocyte differentiation. In the present study, we identified significant gene variants associated with fat deposition (FD)-related processes based on subcutaneous fat tissue RNA-seq data. In the association study, to prove our hypothesis, three Polish pig breeds were included: Złotnicka White (ZW, n = 72), Polish Landrace (PL, n = 201), and Polish Large White (PLW, n = 169). Based on variant calling analysis and χ2 tests, BSCL2 mutations showing significantly different genotype/allele distribution between high- and low-fat pigs were selected for a comprehensive association study. Four interesting BSCL2 variants (rs346079334, rs341493267, rs330154033, and rs81333153) belonging to downstream and missense mutations were investigated. Our study showed a significant decrease in minor allele frequency for two BSCL2 variants (rs346079334 and rs341493267) in PL pigs in 2020–2021. In ZW, BSCL2 mutations significantly affected loin and ham fats, meat redness, and growth performance traits, such as feed conversion and daily feed intake. Similar observations were noted for PLW and PL, where BSCL2 mutations influenced fat depositions and meat traits, such as loin eye area, loin mass and fat, carcass yield, and growth performance traits. Based on the observation in pigs, our study supports the theory that BSCL2 expressed in subcutaneous fat is involved in the FD process.
Funder
Statutory activity of the National Research Institute of Animal Production National Centre for Research and Development in Poland
Subject
General Veterinary,Animal Science and Zoology
Reference39 articles.
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