Sustainable Use of Organic Seaweed Fertilizer Improves the Metagenomic Function of Microbial Communities in the Soil of Rice Plants

Abstract

The frequent use of chemical fertilizers in agricultural practices has developed into a serious environmental concern which urgently needs a solution to restrain their use in agricultural systems. Hence, there is an urgent need to investigate potential organic fertilizers from various natural resources to decrease the use of chemical fertilizers. Seaweed is among the natural resources with potential sustainability value. Our previous work has shown the effectiveness of seaweed fertilizer for increasing plant growth and soil beneficial microbiota. This study aims to evaluate the functional genes present in the soil of rice plants treated with seaweed fertilization. It involves amendments with reduced concentrations of chemical fertilizer in three groups: CF (only chemical fertilizer), CFSF1 (50% dose of CF + seaweed fertilizer 1 ton/ha), and CFSF2 (50% dose of CF + seaweed fertilizer 2 ton/ha). The rice plants supplemented with CFSF1 and CFSF2 were taller and faster to mature compared to CF. In addition, the primary macronutrients nitrogen (N), phosphorus (P), and potassium (K) were also significantly higher in soil supplemented with SF. Our findings showed increased ammonia-oxidizing archaea Crenarchaeota abundance in increasing SF treatments. The PICRUSt analyses indicated enriched functional genes and proteins in relation to amino acid, nucleotide, protein, and carbohydrate metabolism based on the KEGG, BioCyc, and PFAM databases. The current outcomes enhanced our understanding regarding the importance of microbial community for soil quality. Furthermore, seaweed supplementation has shown improvement in soil fertility, which significantly increases rice plant growth and productivity.