Affiliation:
1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
3. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
4. International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, Yangzhou University, Yangzhou 225009, China
Abstract
This study aims to analyze the whole genome sequencing of E. coli F17 in antagonistic and susceptible Hu sheep lambs. The objective is to investigate the critical mutation loci in sheep and understand the genetic mechanism of sheep resistance to E. coli F17 at the genome level. Antagonist and susceptible venous blood samples were collected from Hu sheep lambs for whole genome sequencing and whole genome association analysis. A total of 466 genes with significant SNPs (p < 1.0 × 10−3) were found. GO and KEGG enrichment analysis and protein interaction network analysis were performed on these genes, and preliminary investigations showed that SNPs on CTNNB1, CDH8, APOD, HCLS1, Tet2, MTSS1 and YAP1 genes may be associated with the antagonism and susceptibility of Hu sheep lambs to E. coli F17. There are still some shortcomings that have not been explored via in vivo and in vitro functional experiments of the candidate genes, which will be our next research work. This study provides genetic loci and candidate genes for resistance of Hu sheep lambs to E. coli F17 infection, and provides a genetic basis for breeding disease-resistant sheep.
Funder
National Natural Science Foundation of China-CGIAR
National Natural Science Foundation of China
Major New Varieties of Agricultural Projects in Jiangsu Province
Jiangsu 333 Distinguished Talents Project Foundation
the Open Project Program Of the Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University
Jiangsu Agricultural Science and Technology Innovation Fund
Major Project of Natural Science Foundation of Xinjiang Uyghur Autonomous Region
High-end Foreign Expert Introduction Project
the Natural Science Foundation of the Jiangsu Higher Education Institutions of China
Subject
General Veterinary,Animal Science and Zoology
Reference55 articles.
1. Zhao, X., Zhao, H., Zhou, Z., Miao, Y., Li, R., Yang, B., Cao, C., Xiao, S., Wang, X., and Liu, H. (2022). Characterisation of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates That Cause Diarrhea in Sheep in Northwest China. Microbiol. Spectr., 10.
2. Pathogenic Escherichia coli;Kaper;Nat. Rev. Microbiol.,2004
3. AFA and F17 adhesins produced by pathogenic Escherichia coli strains in domestic animals;Bertin;Vet. Res.,1999
4. Characterization and purification of the F17 adhesin on the surface of bovine enteropathogenic and septicemic Escherichia coli;Lintermans;Am. J. Vet. Res.,1988
5. F17-like fimbriae from an invasive Escherichia coli strain producing cytotoxic necrotizing factor type 2 toxin;Oswald;Infect. Immun.,1994