Metagenomic Sequencing Revealed Differences in the Composition of Cecal Microbes in Different Breeds of Chickens
Author:
Yan Dan12, Li Guohui1, Zhang Huiyong1, Xue Qian1, Zhou Chenghao1, Jiang Yixiu1, Yin Jianmei1, Wang Zhixiu2, Zhao Wenming2, Han Wei1
Affiliation:
1. Jiangsu Institute of Poultry Science, National Chickens Genetic Resources, Yangzhou 225125, China 2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225000, China
Abstract
Intestinal microorganisms are closely related to the health, development, and production performance of animals and can also provide basic information for us to fully understand and explore the germplasm characteristics and traits of local chicken breeds. In this experiment, we used five local Chinese chicken breeds as research materials; metagenomic sequencing technology was used to explore the differences in the composition of intestinal microorganisms in different breeds, and it was found that each local chicken breed had unique unigenes, of which Silkies had the most, with a total of 12,948. GO and KEGG analyses found that the biological processes most enriched by differential functional genes include genetic coding, macromolecular transport, protein synthesis, and molecular functions such as glycoprotein binding, protein hydrolysis, etc. Each breed is enriched with specific pathways, such as Anyi tile-like gray chickens, which are enriched with pathways related to disease resistance, while Gamecocks’ enrichment is related to amino acid metabolism. Random Forest and LEfSe analyses revealed specific species of intestinal microorganisms in the cecum of different breeds, such as Exiguobacterium, which is associated with melanin deposition in Silkies. Therefore, we infer that gut microorganisms are closely related to the formation of chicken breed characteristics, and the results of this experiment can provide a theoretical basis for the discovery of high-quality traits and the improvement of feeding conditions in the future.
Funder
National Key Research and Development Program of China “JBGS” Project of Seed Industry Revitalization in Jiangsu Province
Subject
General Veterinary,Animal Science and Zoology
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