Sensitive and Selective Detection of New Red Colorant Based on Surface-Enhanced Raman Spectroscopy Using Molecularly Imprinted Hydrogels

Abstract

A polyacrylamide-based molecularly imprinted hydrogel (MIH) doped with positively charged gold nanoparticles (Au NPs) has been synthesized via a free radical polymerization of acrylamide (AM) aqueous solution containing positively charged Au NPs as a Raman active substrate, New Red colorant as a template molecule, N,N’-methylenebis(acrylamide) as a crosslinking agent, and potassium persulfate as an initiator. The Au NPs-doped MIHs were subsequently explored as a Raman active substrate for the sensitive and selective detection of New Red colorant via surface-enhanced Raman spectroscopy (SERS). The logarithmic intensity of the characteristic peak of New Red at 1572 cm−1 was proportional to the logarithmic concentration of New Red with a detection linear range of 1.64 × 10−6 to 1.64 × 10−4 M and a limit of detection (LOD) of 1.64 × 10−7 M. The recoveries ranged from 86.3% to 100.6% with a relative standard deviation (RSD) in the range of 2.3% to 7.7%. The RSD and recovery rates for the detection of New Red spiked in a sports drink sample were 1.8% to 7.7% and 91.0% to 97.1%, respectively. These results showed that SERS combined with MIHs as Raman active substrates could provide a sensitive, selective, and effective approach for the detection of the New Red colorant in beverage matrix.