Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?-Reference-Cited by-同舟云学术

Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?

Published:2022-05-16 Issue:5 Volume:14 Page:1060
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Merkuleva Iuliia A.^ORCID,Shcherbakov Dmitry N.^ORCID,Borgoyakova Mariya B.^ORCID,Isaeva Anastasiya A.^ORCID,Nesmeyanova Valentina S.,Volkova Natalia V.,Aripov Vazirbek S.,Shanshin Daniil V.,Karpenko Larisa I.^ORCID,Belenkaya Svetlana V.^ORCID,Kazachinskaia Elena I.,Volosnikova Ekaterina A.,Esina Tatiana I.,Sergeev Alexandr A.^ORCID,Titova Kseniia A.,Konyakhina Yulia V.,Zaykovskaya Anna V.,Pyankov Oleg V.,Kolosova Evgeniia A.,Viktorina Olesya E.,Shelemba Arseniya A.,Rudometov Andrey P.,Ilyichev Alexander A.

Abstract

Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During the first step of assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested the use of five experimental animals (mice, hamsters, rabbits, ferrets, and chickens) for RBD immunogenicity assessments. The humoral immune response was evaluated by ELISA and virus-neutralization assays. The data obtained show hamsters to be the least suitable candidates for RBD immunogenicity testing and, hence, assessing the protective efficacy of RBD-based vaccines.

Funder

Ministry of Science and Higher Education of the Russian Federation

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

Link

https://www.mdpi.com/1999-4915/14/5/1060/pdf

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