Effects of Plant-Based Supplement on Oxidative Stress of Honey Bees (Apis mellifera) Infected with Nosema ceranae

Author:

Jovanovic Nemanja M.1ORCID,Glavinic Uros2ORCID,Ristanic Marko2ORCID,Vejnovic Branislav3ORCID,Ilic Tamara1ORCID,Stevanovic Jevrosima2ORCID,Stanimirovic Zoran2ORCID

Affiliation:

1. Department of Parasitology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, 11000 Belgrade, Serbia

2. Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, 11000 Belgrade, Serbia

3. Department of Economics and Statistics, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, 11000 Belgrade, Serbia

Abstract

One of the most important approaches in the prevention and treatment of nosemosis is the use of herbal preparations as food supplements for bees. Therefore, the aim of this study was to investigate the effects of a plant-based supplement branded as “B+” on honeybees in a laboratory experiment. Four experimental groups were established: treated group (T), N. ceranae-infected and treated group (IT), N. ceranae-infected group (I) and non-infected group (NI). Survival, N. ceranae spore load and oxidative stress parameters together with expression levels of antioxidant enzyme genes and vitellogenin gene were monitored. The mortality in the T, IT and NI groups was significantly (p < 0.001) lower than in than in the I group. Within Nosema-infected groups, the IT group had a significantly lower (p < 0.001) number of N. ceranae spores than the I group. In addition, expression levels of genes for antioxidant enzymes were lower (p < 0.001) in the IT group compared to the I group. The concentration of malondialdehyde and the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) were significantly lower (p < 0.001) in the IT group compared to the I group. No negative effects of the tested supplement were observed. All these findings indicate that the tested supplement exerted beneficial effects manifested in better bee survival, reduced N. ceranae spore number and reduced oxidative stress of bees (lower expression of genes for antioxidant enzymes and oxidative stress parameters).

Funder

Ministry of Science, Technological Development and Innovation of the Republic of Serbia

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

Reference49 articles.

1. Khalifa, S.A., Elshafiey, E.H., Shetaia, A.A., El-Wahed, A.A.A., Algethami, A.F., Musharraf, S.G., AlAjmi, M.F., Zhao, C., Masry, S.H., and Abdel-Daim, M.M. (2021). Overview of bee pollination and its economic value for crop production. Insects, 12.

2. The dependence of crops for pollinators and the economic value of pollination in Brazil;Giannini;J. Econ. Entomol.,2015

3. Lipiński, Z. (2023, October 10). Honey Bee Nutrition and Feeding In Temperate/Continental Climate of the Northern Hemisphere. Published by author, Olsztyn, Poland, 2018, pp 15–137. Available online: https://www.evacranetrust.org/page/honeybee-nutrition-and-feeding.

4. Looking for the causes of and solutions to the issue of honey bee colony losses;Stanimirovic;Acta Vet.—Beograd,2019

5. Plant-based supplement containing B-complex vitamins can improve bee health and increase colony performance;Jovanovic;Prev. Vet. Med.,2021

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