The Effects of Acute Exposure to Ammonia on Oxidative Stress, Hematological Parameters, Flesh Quality, and Gill Morphological Changes of the Large Yellow Croaker (Larimichthys crocea)
Author:
Guo Meijie1, Xu Zhenkun1, Zhang Hongzhi1, Mei Jun123ORCID, Xie Jing123ORCID
Affiliation:
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China 3. Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai 201306, China
Abstract
Ammonia is considered to be the major chemical pollutant causing fish poisoning in aquaculture. This research aimed to evaluate the impact of acute ammonia exposure on the large yellow croaker’s meat quality, gill morphology, liver oxidative stress, and hematological parameters. The fish were exposed to total ammonia nitrogen concentrations of 0, 2.96, 5.92, and 8.87 mg/L for 48 h, respectively. The findings demonstrated that all ammonia-exposed fish had higher liver lactate dehydrogenase and glutamic oxalate transaminase activities. The glucose, blood urea nitrogen, and creatinine levels in 8.87 mg/L total ammonia nitrogen (TAN) were higher than other samples. The total protein, albumin, and triglyceride levels in serum decreased significantly in ammonia-exposed samples. After 48 h of ammonia exposure, superoxide dismutase activities showed a 76.1%, 118.0%, and 156.8% increase when fish were exposed to 2.96, 5.92, and 8.87 mg/L TAN, respectively. Catalase activities and glutathione contents were considerably higher (p < 0.05) in all ammonia-treated samples compared to 0 mg/L TAN. The ammonia-treated gill lamellae become thicker, shorter, and curved. Additionally, the ammonia exposure resulted in the accumulation of free amino acids and the loss of nucleotides. The inosine monophosphate and adenosine monophosphate contents in the flesh were decreased after 12 h of exposure to 2.96, 5.92, and 8.87 mg/L ammonia compared to the control group. Overall, large yellow croakers exposed to ammonia for 6 h presented not only changes in serum composition but also oxidative stress, liver and gill tissue damage and flesh quality deterioration.
Funder
China Agriculture Research System of MOF and MARA Shanghai Municipal Science and Technology Project to Enhance the Capabilities of the PlatForm
Subject
General Veterinary,Animal Science and Zoology
Reference45 articles.
1. Impact of ammonia exposure on coagulation in white shrimp, Litopenaeus vannamei;Chang;Ecotoxicol. Environ. Saf.,2015 2. Wang, Z.L., Guo, X.C., Tu, J., Shi, X., Gan, L., Zhang, M.Z., Jiang, H.B., Zhang, X.X., and Shao, J. (2023). Responses of Micropterus salmoides under Ammonia Stress and the Effects of a Potential Ammonia Antidote. Animals, 13. 3. A Preliminary Study on the Effects of Nitrite Exposure on Hematological Parameters, Oxidative Stress, and Immune-Related Responses in Pearl Gentian Grouper;Zhang;Fishes,2022 4. Lee, J.-W., Jo, A.H., Choi, C.-Y., and Kim, J.-H. (2023). Review of cadmium toxicity effects on fish: Oxidative stress and immune responses. Environ. Res., 116600. 5. Acute exposure to realistic concentrations of Bisphenol-A trigger health damage in fish: Blood parameters, gene expression, oxidative stress;Aquat. Toxicol.,2023
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|