Nutraceutical Potential of Lentinula edodes’ Spent Mushroom Substrate: A Comprehensive Study on Phenolic Composition, Antioxidant Activity, and Antibacterial Effects
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Published:2023-12-15
Issue:12
Volume:9
Page:1200
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ISSN:2309-608X
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Container-title:Journal of Fungi
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language:en
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Short-container-title:JoF
Author:
Baptista Filipa1ORCID, Campos Joana1ORCID, Costa-Silva Valéria2, Pinto Ana Rita1, Saavedra Maria José1ORCID, Ferreira Luis Mendes1ORCID, Rodrigues Miguel1ORCID, Barros Ana Novo1ORCID
Affiliation:
1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal 2. CECAV—Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Abstract
Lentinula edodes, commonly known as shiitake mushroom, is renowned for its potential health advantages. This research delves into the often-overlooked by-product of shiitake cultivation, namely spent mushroom substrate (SMS), to explore its nutraceutical properties. The SMS samples were collected and subjected to different extraction methods, namely short or long agitation, and ultrasound-assisted extractions using different temperatures and distilled water or a 50% (v/v) ethanol as solvents. The extracts were tested for phenolic content (total phenols, ortho-diphenols, and flavonoids), antioxidant capacity (DPPH, 2,2-diphenyl-1 picrylhydrazyl; ABTS, 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid; and FRAP, ferric reducing antioxidant power), and antibacterial activity. The different extraction methods revealed substantial variations (p < 0.05) in phenolic composition and antioxidant capacity. The highest phenolic content and antioxidant capacity were achieved using 24 h extraction, agitation, 50 °C, and ethanol as the solvent. Furthermore, the extracted compounds displayed antibacterial activity in specific tested bacterial strains. This study highlights the nutraceutical potential of L. edodes’ SMS, positioning it as a valuable dietary supplement for animal nutrition, with emphasis on its prebiotic properties. Hence, this research unveils the promising health benefits of SMS in both human and animal nutrition.
Funder
FCT—Portuguese Foundation for Science and Technology HORIZON-RIA
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
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