Understanding Mutations in Human SARS-CoV-2 Spike Glycoprotein: A Systematic Review & Meta-Analysis

Author:

Kumar Reetesh12ORCID,Srivastava Yogesh3,Muthuramalingam Pandiyan4ORCID,Singh Sunil Kumar5,Verma Geetika2ORCID,Tiwari Savitri6ORCID,Tandel Nikunj7ORCID,Beura Samir Kumar5,Panigrahi Abhishek Ramachandra5ORCID,Maji Somnath8ORCID,Sharma Prakriti9,Rai Pankaj Kumar10ORCID,Prajapati Dinesh Kumar10,Shin Hyunsuk4ORCID,Tyagi Rajeev K.9ORCID

Affiliation:

1. Faculty of Agricultural Sciences, Institute of Applied Sciences & Humanities, GLA University, Mathura 281406, India

2. Department of Biotherapeutics, CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160036, India

3. Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA

4. Division of Horticultural Science, Gyeongsang National University, Jinju 52725, Republic of Korea

5. Department of Zoology, School of Biological Sciences, Central University of Punjab, Ghudda, Bathinda 151401, India

6. Division of Life Sciences, Department of Biosciences, School of Basic and Applied Sciences, Galgotias University, Gautam Buddha Nagar, Greater Noida 201310, India

7. Institute of Science, Nirma University, SG Highway, Gujarat 382481, India

8. Department of Radiology, University of Michigan, Ann Arbor, MI 48109, USA

9. Biomedical Parasitology and Translational-Immunology Lab, CSIR-Institute of Microbial Technology (IMTECH), Chandigarh 160036, India

10. Department of Biotechnology, IIET, Invertis University, Bareilly 243001, India

Abstract

Genetic variant(s) of concern (VoC) of SARS-CoV-2 have been emerging worldwide due to mutations in the gene encoding spike glycoprotein. We performed comprehensive analyses of spike protein mutations in the significant variant clade of SARS-CoV-2, using the data available on the Nextstrain server. We selected various mutations, namely, A222V, N439K, N501Y, L452R, Y453F, E484K, K417N, T478K, L981F, L212I, N856K, T547K, G496S, and Y369C for this study. These mutations were chosen based on their global entropic score, emergence, spread, transmission, and their location in the spike receptor binding domain (RBD). The relative abundance of these mutations was mapped with global mutation D614G as a reference. Our analyses suggest the rapid emergence of newer global mutations alongside D614G, as reported during the recent waves of COVID-19 in various parts of the world. These mutations could be instrumentally imperative for the transmission, infectivity, virulence, and host immune system’s evasion of SARS-CoV-2. The probable impact of these mutations on vaccine effectiveness, antigenic diversity, antibody interactions, protein stability, RBD flexibility, and accessibility to human cell receptor ACE2 was studied in silico. Overall, the present study can help researchers to design the next generation of vaccines and biotherapeutics to combat COVID-19 infection.

Funder

DBT, New Delhi, Ramalingaswami Re-entry Fellowship Project

Indian Council of Medical Research

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

Reference108 articles.

1. COVID-19: Transmission, prevention, and potential therapeutic opportunities;Lotfi;Clin. Chim. Acta.,2020

2. WHO (2020). Coronavirus Disease 2019 (COVID-19): Situation Report, WHO.

3. SARS-CoV-2 spike protein: Pathogenesis, vaccines, and potential therapies;Almehdi;Infection,2021

4. Characteristics of SARS-CoV-2 and COVID-19;Hu;Nat. Rev. Microbiol.,2021

5. Mechanisms of SARS-CoV-2 entry into cells;Jackson;Nat. Rev. Mol. Cell Biol.,2022

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3