Affiliation:
1. PKDERM Laboratories, 06130 Grasse, France
2. Probiotic Group Luxembourg S.A., Research and Development Department, 9944 Weiswampach, Luxembourg
Abstract
The use of probiotics has gained increasing attention as a strategy for wound healing to decrease microbial resistance to disinfectants and antibiotics. This study aimed to investigate the potential of a non-medicinal topical cocktail of probiotic bacteria (CPB) in promoting wound healing in dogs using in vitro scratch assay. Canine Progenitors Epidermal Keratinocytes (CPEK) were exposed to a prototype product formulated with CPB (PPP), non-formulated CPB, and the vehicle. The viability of CPB and CPEK cells was first evaluated in the co-culture model. Then, wound closure was analyzed over time. The CPB required a minimum concentration of 75 CFU/mL for better viability with CPEK. While the CPEK preserved 100% of their viability when PPP was diluted to up to 75,000 CFU/mL. At higher concentrations, the viability of CPEK was reduced by the concomitant effect of the non-formulated CPB and the vehicle. The formulated and non-formulated CPB and the vehicle seem to lead to a dose-dependent increase in cell migration compared to the control. Importantly, at the concentration of 750,000 CFU/mL, the PPP showed a 20% increase in wound closure. Taken together, our findings suggest the potential beneficial effects of the probiotic-based topical cocktail (PPP) on wound healing. However, to confirm and validate these effects, further experiments are necessary to provide more robust evidence and allow us to confidently establish the potential beneficial effects of the probiotic bacteria (CPB) in promoting wound healing.
Funder
Government of the Grand Duchy of Luxembourg I Ministry of the Economy
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference32 articles.
1. Principles of Wound Management and Wound Healing in Exotic Pets;Mickelson;Vet. Clin. N. Am. Exot. Anim. Pract.,2016
2. Wound healing in animals: A review of physiology and clinical evaluation;Lux;Vet. Dermatol.,2022
3. Wound Healing: A Comprehensive Review;Almadani;Semin. Plast. Surg.,2021
4. Living Bacterial Hydrogels for Accelerated Infected Wound Healing;Ming;Adv. Sci.,2021
5. Togo, C., Zidorio, A.P., Gonçalves, V., Botelho, P., de Carvalho, K., and Dutra, E. (2021). Does Probiotic Consumption Enhance Wound Healing? A Systematic Review. Nutrients, 14.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献