Triangular Silver Nanoplates as a Bioanalytical Tool: Potential COVID-19 Detection
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Published:2023-07-26
Issue:15
Volume:24
Page:11974
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Rodriguez Barroso Laura G.1, Lanzagorta Garcia Eduardo1ORCID, Mojicevic Marija1ORCID, Alkan Tas Buket1, Huerta Miriam2ORCID, Pogue Robert3, Devine Declan M.1ORCID, Brennan-Fournet Margaret1
Affiliation:
1. PRISM Research Institute, Technological University of the Shannon: Midlands Midwest, Dublin Rd, N37 HD68 Athlone, Ireland 2. Physics Institute, Universidad Autónoma de San Luis Potosí, Av. Parque Chapultepec 1570, San Luis Potosí 78295, Mexico 3. Campus Asa Norte, Universidade Católica de Brasília, SGAN Módulo B 916 Avenida W5, Brasilia 70790-160, Brazil
Abstract
Nanotechnology offers new possibilities in molecular diagnostics, with nanoparticles gaining attention as biosensor upgrades. This study evaluates gold-coated silver nanoplates coated with PEG for enhanced protection, aiming to detect Spike protein with higher sensitivity, and emphasizes the importance of considering complex environments and appropriate controls for specific binding and accurate analysis. The sensitivity of antibody-coated PEGAuTSNPs as tools for immunoassays is demonstrated through fibronectin (Fn)– anti-fibronectin binding within an isolated extracellular matrix as a complex and native environment of Fn. Moreover, the optimal functionalization volume of Spike protein was determined (4 µg/mL of PEGAuTSNP). Anti-Spike was added to confirm binding, while the TJP1 protein was used as a negative control. The same experiment was used in the presence of horse serum to simulate a complex environment. According to Localized Surface Plasmon Resonance analysis and Dynamic Light Scattering size measurements, anti-Spike exhibited a stronger affinity for the nanoplates, causing TJP1 to be replaced by the antibody on the nanoplates’ surface. Future research will involve exploring alternative complex environments, filtering larger molecules, and the optimization of immunoassay performance.
Funder
Technological University of The Shannon
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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