Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis

Abstract

An interspaced repeat sequence (IRS) is a unique sequence similar to prokaryotic CRISPR in structure. In this study, 1343 IRSs were identified in the Arabidopsis genome. Functional annotation of the IRS-related genes showed that they were associated with various growth and development processes. More than 30% of the IRSs were located in promoter regions. Deletion of some IRSs affected promoter activity, suggesting their roles in the regulation of gene expression. Next, the function of the AtXTH10 gene was further analyzed, and the expression of this gene was regulated by IRSs in its promoter region. Transgenic and mutant plants analysis indicated that the AtXTH10 gene was associated with root development by affecting cell wall structure. Moreover, the expression profiles of some key genes involved in root development signaling pathways were also affected by AtXTH10. These results suggest that IRSs could be involved in regulating the expression of genes with important roles in plant development.