Comparison of the Phenotypic Performance, Molecular Diversity, and Proteomics in Transgenic Rice
Author:
Sun Yue,Zhao Huan,Chen Zhongkai,Chen Huizhen,Li Bai,Wang Chunlei,Lin Xiaoli,Cai Yicong,Zhou Dahu,Ouyang Linjuan,Zhu Changlan,He Haohua,Peng Xiaosong
Abstract
The extent of molecular diversity and differentially expressed proteins (DEPs) in transgenic lines provide valuable information to understand the phenotypic performance of transgenic crops compared with their parents. Here, we compared the differences in the phenotypic variation of twelve agronomic and end-use quality traits, the extent of microsatellite diversity, and DEPs of a recurrent parent line with three transgenic rice restorer lines carrying either CRY1C gene on chromosome 11 or CRY2A gene on chromosome 12 or both genes. The three transgenic lines had significantly smaller stem borer infestation than the recurrent parent without showing significant differences among most agronomic traits, yield components, and end-use quality traits. Using 512 microsatellite markers, the three transgenic lines inherited 2.9–4.3% of the Minghui 63 donor genome and 96.3–97.1% of the CH891 recurrent parent genome. As compared with the recurrent parent, the number of upregulated and down-regulated proteins in the three transgenic lines varied from 169 to 239 and from 131 to 199, respectively. Most DEPs were associated with the secondary metabolites biosynthesis transport and catabolism, carbohydrate transport and metabolism, post-translational modification, and signal transduction mechanisms. Although several differentially expressed proteins were observed between transgenic rice and its recurrent parent, the differences may not have been associated with grain yield and most other phenotypic traits in transgenic rice.
Funder
the Major Project supported by the Major Project of Jiangxi Provincial Department of Science and Technology
the Major Project supported by Breeding New Varieties of the National Transgene of China
Jiangxi Provincial Natural Science Foundation of China
Jiangxi Provincial Major Science and Technology Research Project
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics