Integrated Transcriptome and Metabolome Analyses Reveal Details of the Molecular Regulation of Resistance to Stem Nematode in Sweet Potato
Author:
Qiao Shouchen1ORCID,
Ma Jukui2,
Wang Yannan1,
Chen Jingwei2,
Kang Zhihe1,
Bian Qianqian1,
Chen Jinjin1,
Yin Yumeng1,
Cao Guozheng1,
Zhao Guorui1,
Yang Guohong1,
Sun Houjun2,
Yang Yufeng1
Affiliation:
1. Cereal Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
2. Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai Area, Xuzhou 221000, China
Abstract
Stem nematode disease can seriously reduce the yield of sweet potato (Ipomoea batatas (L.) Lam). To explore resistance mechanism to stem nematode in sweet potato, transcriptomes and metabolomes were sequenced and compared between two sweet potato cultivars, the resistant Zhenghong 22 and susceptible Longshu 9, at different times after stem nematode infection. In the transcriptional regulatory pathway, mitogen-activated protein kinase signaling was initiated in Zhenghong 22 at the early stage of infection to activate genes related to ethylene production. Stem nematode infection in Zhenghong 22 also triggered fatty acid metabolism and the activity of respiratory burst oxidase in the metabolic pathway, which further stimulated the glycolytic and shikimic pathways to provide raw materials for secondary metabolite biosynthesis. An integrated analysis of the secondary metabolic regulation pathway in the resistant cultivar Zhenghong 22 revealed the accumulation of tryptophan, phenylalanine, and tyrosine, leading to increased biosynthesis of phenylpropanoids and salicylic acid and enhanced activity of the alkaloid pathway. Stem nematode infection also activated the biosynthesis of terpenoids, abscisic acid, zeatin, indole, and brassinosteroid, resulting in improved resistance to stem nematode. Finally, analyses of the resistance regulation pathway and a weighted gene co-expression network analysis highlighted the importance of the genes itf14g17940 and itf12g18840, encoding a leucine-rich receptor-like protein and 1-aminocyclopropane-1-carboxylate synthase, respectively. These are candidate target genes for increasing the strength of the defense response. These results provide new ideas and a theoretical basis for understanding the mechanism of resistance to stem nematode in sweet potato.
Funder
Independent Innovation Program of Henan Academy of Agricultural Sciences
Earmarked Fund for CARS-10-Sweet Potato
Special Fund for Henan Agriculture Research System
Henan Joint Research Program for Improved Agricultural Varieties
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
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