Affiliation:
1. College of Animal Science and Technology, Southwest University, Chongqing 402460, China
2. Bazhong Academy of Agriculture and Forestry Sciences, Bazhong 636099, China
3. Institute of Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China
4. Chongqing Key Laboratory of Herbivore Science, Chongqing 402460, China
Abstract
The sorghum–sudangrass hybrid is the main high-quality forage grass in Southwest China, but, in recent years, it has suffered from leaf spot disease, with a prevalence of 88% in Bazhong, Sichuan, China, seriously affecting yield and quality. The causal agents were obtained from symptomatic leaves by tissue isolation and verified by pathogenicity assays. A combination of morphological characterization and sequence analysis revealed that strains SCBZSL1, SCBZSX5, and SCBZSW6 were Nigrospora sphaerica, Colletotrichum boninense, and Didymella corylicola, respectively, and the latter two were the first instance to be reported on sorghum–sudangrass hybrids in the world. SCBZSX5 significantly affected the growth of the plants, which can reduce plant height by 25%. The biological characteristics of SCBZSX5 were found to be less sensitive to the change in light and pH, and its most suitable culture medium was Potato Dextrose Agar (PDA), with the optimal temperature of 25 °C and lethal temperature of 35 °C. To clarify the interactions between the pathogen SCBZSX5 and plants, metabolomics analyses revealed that 211 differential metabolites were mainly enriched in amino acid metabolism and flavonoid metabolism. C. boninense disrupted the osmotic balance of the plant by decreasing the content of acetyl proline and caffeic acid in the plant, resulting in disease occurrence, whereas the sorghum–sudangrass hybrids improved tolerance and antioxidant properties through the accumulation of tyrosine, tryptophan, glutamic acid, leucine, glycitein, naringenin, and apigetrin to resist the damage caused by C. boninense. This study revealed the mutualistic relationship between sorghum–sudangrass hybrids and C. boninense, which provided a reference for the control of the disease.
Funder
Chongqing Natural Science Foundation
National Training Program of Innovation and Entrepreneurship for Undergraduates
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Cited by
1 articles.
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