Abstract
We generated an orange-colored (OC) rice callus line by targeted mutagenesis of the orange gene (OsOr) using the CRISPR-Cas9 system. The OC line accumulated more lutein, β-carotene, and two β-carotene isomers compared to the WT callus line. We also analyzed the expression levels of carotenoid biosynthesis genes by qRT-PCR. Among the genes encoding carotenoid metabolic pathway enzymes, the number of transcripts of the PSY2, PSY3, PDS, ZDS and β-LCY genes were higher in the OC line than in the WT line. In contrast, transcription of the ε-LCY gene was downregulated in the OC line compared to the WT line. In addition, we detected increases in the transcript levels of two genes involved in carotenoid oxidation in the OC lines. The developed OC lines also showed increased tolerance to salt stress. Collectively, these findings indicate that targeted mutagenesis of the OsOr gene via CRISPR/Cas9-mediated genome editing results in β-carotene accumulation in rice calli. Accordingly, we believe that this type of genome-editing technology could represent an effective alternative approach for enhancing the β-carotene content of plants.
Funder
New Breeding Technologies Development Program
Ministry of Education, Republic of Korea
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference49 articles.
1. Chemistry, stability, and biological actions of carotenoids;Yahia;Fruit and Vegetable Phytochemicals: Chemistry and Human Health,2017
2. Young, A.J., and Pallett, K.E. (2017). Antioxidants in HIGHER Plants, CRC Press.
3. Effects of carotenoids on human immune function;Hughes;Proc. Nutr. Soc.,1999
4. Source to sink: Regulation of carotenoid biosynthesis in plants;Cazzonelli;Trends Plant Sci.,2010
5. Downregulation of the lycopene ε-cyclase gene increases carotenoid synthesis via the β-branch-specific pathway and enhances salt-stress tolerance in sweetpotato transgenic calli;Kim;Physiol. Plant.,2013
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献