Peptidome and Transcriptome Analysis of Plant Peptides Involved in Bipolaris maydis Infection of Maize-Reference-Cited by-同舟云学术

Peptidome and Transcriptome Analysis of Plant Peptides Involved in Bipolaris maydis Infection of Maize

Published:2023-03-14 Issue:6 Volume:12 Page:1307
ISSN:2223-7747
Container-title:Plants
language:en
Short-container-title:Plants

Author:

Sheng Pijie¹²,Xu Minyan¹²,Zheng Zhenzhen¹,Liu Xiaojing¹,Ma Wanlu¹²,Ding Ting³,Zhang Chenchen¹²,Chen Meng¹²,Zhang Mengting¹,Cheng Beijiu¹²,Zhang Xin¹²

Affiliation:

1. National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China

2. Key Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China

3. School of Plant Protection, Anhui Agricultural University, Hefei 230036, China

Abstract

Southern corn leaf blight (SCLB) caused by Bipolaris maydis threatens maize growth and yield worldwide. In this study, TMT-labeled comparative peptidomic analysis was established between infected and uninfected maize leaf samples using liquid-chromatography-coupled tandem mass spectrometry. The results were further compared and integrated with transcriptome data under the same experimental conditions. Plant peptidomic analysis identified 455 and 502 differentially expressed peptides (DEPs) in infected maize leaves on day 1 and day 5, respectively. A total of 262 common DEPs were identified in both cases. Bioinformatic analysis indicated that the precursor proteins of DEPs are associated with many pathways generated by SCLB-induced pathological changes. The expression profiles of plant peptides and genes in maize plants were considerably altered after B. maydis infection. These findings provide new insights into the molecular mechanisms of SCLB pathogenesis and offer a basis for the development of maize genotypes with SCLB resistance.

Funder

the Key Program of Joint Funds of the National Natural Science Foundation of China

the Major Scientific and Technological Project in Anhui Province

the Scientific Research Project of Department of Education of Anhui Province

Publisher

MDPI AG

Subject

Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics

Link

https://www.mdpi.com/2223-7747/12/6/1307/pdf

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