Systemic Signals Induced by Single and Combined Abiotic Stimuli in Common Bean Plants

Abstract

To survive in a dynamic environment growing fixed to the ground, plants have developed mechanisms for monitoring and perceiving the environment. When a stimulus is perceived, a series of signals are induced and can propagate away from the stimulated site. Three distinct types of systemic signaling exist, i.e., (i) electrical, (ii) hydraulic, and (iii) chemical, which differ not only in their nature but also in their propagation speed. Naturally, plants suffer influences from two or more stimuli (biotic and/or abiotic). Stimuli combination can promote the activation of new signaling mechanisms that are explicitly activated, as well as the emergence of a new response. This study evaluated the behavior of electrical (electrome) and hydraulic signals after applying simple and combined stimuli in common bean plants. We used simple and mixed stimuli applications to identify biochemical responses and extract information from the electrical and hydraulic patterns. Time series analysis, comparing the conditions before and after the stimuli and the oxidative responses at local and systemic levels, detected changes in electrome and hydraulic signal profiles. Changes in electrome are different between types of stimulation, including their combination, and systemic changes in hydraulic and oxidative dynamics accompany these electrical signals.