Characterization of the Chemopreventive Properties of Cannabis sativa L. Inflorescences from Monoecious Cultivars Grown in Central Italy
Author:
Di Giacomo Silvia12ORCID, Percaccio Ester1ORCID, Vitalone Annabella1ORCID, Ingallina Cinzia3ORCID, Mannina Luisa3ORCID, Macone Alberto4ORCID, Di Sotto Antonella1ORCID
Affiliation:
1. Department of Physiology and Pharmacology “V. Erspamer”, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy 2. Unit of Human Nutrition and Health, Department of Food Safety, Nutrition and Veterinary Public Health, National Institute of Health, 00161 Rome, Italy 3. Department of Drug Chemistry and Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy 4. Department of Biochemical Science “A. Rossi Fanelli”, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Abstract
Hemp bioproducts hold great promise as valuable materials for nutraceutical and pharmaceutical applications due to their diverse bioactive compounds and potential health benefits. In line with this interest and in an attempt to valorize the Lazio Region crops, this present study investigated chemically characterized hydroalcoholic and organic extracts, obtained from the inflorescences of locally cultivated Felina 32, USO 31, Ferimon and Fedora 17 hemp varieties. In order to highlight the possible chemopreventive power of the tested samples, a bioactivity screening was performed, which included studying the antimutagenic activity, radical scavenging power, cytotoxicity in human hepatoma HepG2 cells, leakage of lactate dehydrogenase (LDH) and modulation of the oxidative stress parameters and glucose-6-phosphate dehydrogenase (G6PDH) involved in the regulation of the cell transformation and cancer proliferation. Tolerability studies in noncancerous H69 cholangiocytes were performed, too. The organic extracts showed moderate to strong antimutagenic activities and a marked cytotoxicity in the HepG2 cells, associated with an increased oxidative stress and LDH release, and to a G6PDH modulation. The hydroalcoholic extracts mainly exhibited radical scavenging properties with weak or null activities in the other assays. The extracts were usually well-tolerated in H69 cells, except for the highest concentrations which impaired cell viability, likely due to an increased oxidative stress. The obtained results suggest a possibility in the inflorescences from the Felina 32, USO 31, Ferimon and Fedora 17 hemp varieties as source of bioactive compounds endowed with genoprotective and chemopreventive properties that could be harnessed as preventive or adjuvant healing strategies.
Funder
Regione Lazio Sapienza University
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference87 articles.
1. Visković, J., Zheljazkov, V.D., Sikora, V., Noller, J., Latković, D., Ocamb, C.M., and Koren, A. (2023). Industrial Hemp (Cannabis sativa L.) Agronomy and Utilization: A Review. Agronomy, 13. 2. Cannabis and Cannabis Edibles: A Review;Peng;J. Agric. Food Chem.,2021 3. Medical Cannabis: A plurimillennial history of an evergreen;Pisanti;J. Cell Physiol.,2019 4. Placido, D.F., and Lee, C.C. (2022). Potential of Industrial Hemp for Phytoremediation of Heavy Metals. Plants, 11. 5. Testa, G., Corinzia, S.A., Cosentino, S.L., and Ciaramella, B.R. (2023). Phytoremediation of Cadmium-, Lead-, and Nickel-Polluted Soils by Industrial Hemp. Agronomy, 13.
|
|