In Vitro Antimicrobial Activity of Nymphaea pubescens (Pink Water Lily) Leaf Extracts

Author:

Thongdonphum Boontarika1ORCID,Vanichkul Kittima1ORCID,Bunchaleamchai Adun2,Powthong Pannapa3ORCID

Affiliation:

1. Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Thanyaburi 12130, Thailand

2. Faculty of Medical Technology, Rangsit University, Mueang Pathum Thani 12000, Thailand

3. Faculty of Science, Rangsit University, Mueang Pathum Thani 12000, Thailand

Abstract

This research comparatively investigates the in vitro antimicrobial activity of extracts from Nymphaea pubescens (pink water lily) leaves against pathogenic bacteria. The experimental extracts are aqueous, acetonic, and 95% ethanolic N. pubescens extracts; and the pathogenic bacteria being studied include Aeromonas hydrophila, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio harveyi, which are commonly found in freshwater fish and brackish aquatic animals. The ethanolic N. pubescens extract achieves the highest bacterial inhibitory effects against V. parahaemolyticus and V. vulnificus. The minimum inhibitory concentrations of the ethanolic extract against A. hydrophila and V. harveyi are 10 mg/mL; and 2.5 mg/mL against V. parahaemolyticus and V. vulnificus. The ethanolic N. pubescens extract is effective against V. parahaemolyticus. The high-performance liquid chromatography results show that, in the phenolic acids group, gallic acid is the most dominant (0.600–3.21% w/w), followed by sinapic acid (0.37–0.83% w/w). In the flavonoids group, catechin is the most dominant (0.02–1.08% w/w), followed by rutin (0.002–0.03% w/w). Essentially, the ethanolic N. pubescens extract can potentially be used as a natural antibiotic agent to treat bacterial infections in fish and aquatic animals.

Funder

Science, Research, and Innovation Promotion Funding

Rajamangala University of Technology Thanyaburi

Publisher

MDPI AG

Subject

Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics

Reference34 articles.

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3. Sumpradit, N., Suttajit, S., Poonpolsub, S., Chuanchuen, R., and Prakongsai, P. (2023, August 01). Landscape of Antimicrobial Resistance Situation and Action in Thailand. Available online: http://narst.dmsc.moph.go.th/documentation/AMR%20strategy%202560-2564.pdf.

4. Parimala, M., and Shoba, F.G. (2014). In vitro antimicrobial activity and HPTLC analysis of hydroalcoholic seed extract of Nymphaea nouchali Burm.f. BMC Complement. Altern. Med., 14.

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