Physiological Response of Nutrient-Stressed Lemna gibba to Pulse Colloidal Silver Treatment

Abstract

Wastewater is a source of many environmental pollutants and potentially high concentrations of essential plant nutrients. Site-specific nutrient levels may influence the response of exposed plants to a chemical stressor. In the present study, we focused on the responses of model aquatic macrophyte swollen duckweed (Lemna gibba L.) to a short pulse exposure and a commercially available colloidal silver product as a potential environmental chemical stressor, combined with two levels of total nitrogen and phosphorus nutrition. Treatment with the commercially available colloidal silver product caused oxidative stress in L. gibba plants under both high and low nutrient levels. Plants grown and treated under high nutrient levels showed lower levels of lipid peroxidation and hydrogen peroxide accumulation, as well as higher levels of photosynthetic pigment content in comparison to treated plants under low nutrient levels. Higher free radical scavenging activity for plants treated with silver in combination with high nutrient levels resulted in better overall protection from silver-induced oxidative stress. The results showed that external nutrient levels significantly affected the L. gibba plant’s response to the colloidal silver presence in the environment and that nutrient levels should be considered in the assessment of potential environmental impact for contaminants.