Coordination of m6A mRNA Methylation and Gene Transcriptome in Sugarcane Response to Drought Stress
Author:
Wei Jinju12, Li Haibi3ORCID, Gui Yiyun12, Zhou Hui12, Zhang Ronghua12, Zhu Kai12, Liu Xihui12
Affiliation:
1. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China 2. Guangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, Nanning 530007, China 3. Guangxi South Subtropical Agricultural Science Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 532415, China
Abstract
The N6-methyladenosine (m6A) methylation of mRNA is involved in biological processes essential for plant growth. To explore the m6A modification of sugarcane and reveal its regulatory function, methylated RNA immunoprecipitation sequencing (MeRIP-seq) was used to construct the m6A map of sugarcane. In this study, m6A sites of sugarcane transcriptome were significantly enriched around the stop codon and within 3′-untranslated regions (3′UTR). Gene ontology (GO) analysis showed that the m6A modification genes are associated with metabolic biosynthesis. In addition, the m6A modification of drought-resistant transcript mRNA increased significantly under drought (DR) treatment, resulting in enhanced mRNA stability, which is involved in regulating sugarcane drought resistance. GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results showed that differentially methylated peak (DMP) modification of differentially expressed genes (DEGs) in DR were particularly associated with abscisic acid (ABA) biosynthesis. The upregulated genes were significantly enriched in the ABA metabolism, ethylene response, fatty acid metabolism, and negative regulation of the abscisic acid activation signaling pathway. These findings provide a basis and resource for sugarcane RNA epigenetic studies and further increase our knowledge of the functions of m6A modifications in RNA under abiotic stress.
Funder
National Natural Science Foundation of China Guangxi Natural Science Foundation Foundation for Guangxi Academy of Agricultural Sciences CARS
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
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