Integrative Metabolic and Transcriptomic Profiling in Camellia oleifera and Camellia meiocarpa Uncover Potential Mechanisms That Govern Triacylglycerol Degradation during Seed Desiccation

Abstract

Camellia seed oil is a top-end quality of cooking oil in China. The oil quality and quantity are formed during seed maturation and desiccation. So far, it remains largely unresolved whether lipid degradation occurs and contributes to Camellia oil traits. In this study, three different Camellia germplasms, C. oleifera cv. Min 43 (M43), C. meiocarpa var. Qingguo (QG), and C. meiocarpa cv Hongguo (HG) were selected, their seed oil contents and compositions were quantified across different stages of seed desiccation. We found that at the late stage of desiccation, M43 and QG lost a significant portion of seed oil, while such an event was not observed in HG. To explore the molecular bases for the oil loss In M43, the transcriptomic profiling of M43 and HG was performed at the early and the late seed desiccation, respectively, and differentially expressed genes (DEGs) from the lipid metabolic pathway were identified and analyzed. Our data demonstrated that different Camellia species have diverse mechanisms to regulate seed oil accumulation and degradation, and that triacylglycerol-to-terpenoid conversion could account for the oil loss in M43 during late seed desiccation.