Affiliation:
1. Departamento de Química, Universidad Técnica Particular de Loja (UTPL), Calle Marcelino Champagnat s/n, Loja 110107, Ecuador
2. Departamento de Ciencias Biológicas y Agropecuarias, Universidad Técnica Particular de Loja (UTPL), Calle Marcelino Champagnat s/n, Loja 110107, Ecuador
Abstract
An essential oil, distilled from the leaves of the Andean species Gynoxys rugulosa Muschl., is described in the present study for the first time. The chemical composition was qualitatively and quantitatively determined by GC–MS and GC–FID, respectively. On the one hand, the qualitative composition was obtained by comparing the mass spectrum and the linear retention index of each component with data from literature. On the other hand, the quantitative composition was determined by calculating the relative response factor of each constituent, according to its combustion enthalpy. Both analyses were carried out with two orthogonal columns of nonpolar and polar stationary phases. A total of 112 compounds were detected and quantified with at least one column, corresponding to 87.3–93.0% of the whole oil mass. Among the 112 detected components, 103 were identified. The main constituents were α-pinene (5.3–6.0%), (E)-β-caryophyllene (2.4–2.8%), α-humulene (3.0–3.2%), germacrene D (4.9–6.5%), δ-cadinene (2.2–2.3%), caryophyllene oxide (1.6–2.2%), α-cadinol (3.8–4.4%), 1-nonadecanol (1.7–1.9%), 1-eicosanol (0.9–1.2%), n-tricosane (3.3–3.4%), 1-heneicosanol (4.5–5.8%), n-pentacosane (5.8–7.1%), 1-tricosanol (4.0–4.5%), and n-heptacosane (3.0–3.5%). Furthermore, an enantioselective analysis was carried out on the essential oil, by means of two cyclodextrin-based capillary columns. The enantiomers of α-pinene, β-pinene, sabinene, α-phellandrene, β-phellandrene, linalool, α-copaene, terpinen-4-ol, α-terpineol, and germacrene D were detected, and the respective enantiomeric excess was calculated.
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference111 articles.
1. Phytochemistry and Ethnopharmacology of the Ecuadorian Flora. A Review;Andrade;Nat. Prod. Commun.,2016
2. Armijos, C., Ramírez, J., Salinas, M., Vidari, G., and Suárez, A.I. (2021). Pharmacology and Phytochemistry of Ecuadorian Medicinal Plants: An Update and Perspectives. Pharmaceuticals, 14.
3. (2022, November 17). Megadiverse Countries, UNEP-WCMC. Available online: https://www.biodiversitya-z.org/content/megadiverse-countries.
4. New Anthracene Derivatives from Coussarea macrophylla;Chiriboga;J. Nat. Prod.,2003
5. Anti-secretory, Anti-inflammatory, and Anti-Helicobacter pylori Activities of Several Fractions Isolated from Piper carpunya Ruiz & Pav;Berenguer;J. Ethnopharmacol.,2010