Glyphosate-Induced Abscisic Acid Accumulation Causes Male Sterility in Sea Island Cotton
Author:
Qin Guoli12ORCID, Zhao Nan3ORCID, Wang Weiran2, Wang Meng2, Zhu Jiahui2, Yang Jing24, Lin Feng12, Huang Xinglei12, Zhang Yanhui5, Min Ling4ORCID, Chen Guodong1ORCID, Kong Jie26ORCID
Affiliation:
1. College of Agriculture, Tarim University, Alar 843300, China 2. Institute of Cash Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China 3. College of Agronomy and Biotechnology, China Agricultusral University, Beijing 100000, China 4. Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China 5. College of Grassland Sciences, Xinjiang Agricultural University, Urumqi 830052, China 6. The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Urumqi 830052, China
Abstract
Sea Island cotton is the best quality tetraploid cultivated cotton in the world, in terms of fiber quality. Glyphosate is a widely used herbicide in cotton production, and the improper use of herbicides has led to pollen abortion in sea island cotton and, consequently, to a dramatic decrease in yield; however, the mechanism remains unclear. In this study, different concentrations (0, 3.75, 7.5, 15, and 30 g/L) of glyphosate were applied to CP4-EPSPS transgenic sea island cotton Xinchang 5 in 2021 and 2022 at Korla, with 15 g/L glyphosate chosen as the suitable concentration. By comparing the paraffin sections of 2–24 mm anthers in the 15 g/L glyphosate treatment group with those in the water control group, we showed that the key period of anther abortion after glyphosate treatment was the formation and development of tetrads, which corresponded to 8–9 mm buds. Transcriptome sequencing analysis of the treated and control anthers revealed a significant enrichment of differentially expressed genes in phytohormone-related pathways, in particular abscisic acid response and regulation pathways. Additionally, after treatment with 15 g/L of glyphosate, there was a significant increase in the amount of abscisic acid in the anthers in the 8–9 mm buds. Further analysis of the differential expression of abscisic acid response and regulatory genes, an abscisic acid response gene GbTCP14 (Gbar_A11G003090) was identified, which was significantly upregulated in buds with 15 g/L glyphosate treatment than the control, and it could be a key candidate gene for the subsequent research involving male sterility induced by glyphosate in sea island cotton.
Funder
Xinjiang Academy of Agricultural Sciences Stable Support to Agricultural Sci-Tech Renovation China Agriculture Research System Xinjiang Youth Science Fund General Program
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference55 articles.
1. Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadenseand identifying QTLs for lint percentage;Shi;J. Integr. Plant Biol.,2014 2. Su, X., Zhu, G., Song, X., Xu, H., Li, W., Ning, X., Chen, Q., and Guo, W. (2020). Genome-wide association analysis reveals loci and candidate genes involved in fiber quality traits in sea island cotton (Gossypium barbadense). BMC Plant Biol., 20. 3. Roberts, J., Florentine, S., Fernando, W.G.D., and Tennakoon, K.U. (2022). Achievements, Developments and Future Challenges in the Field of Bioherbicides for Weed Control: A Global Review. Plants, 11. 4. Effect of glyphosate application timings and methods on glyphosate-resistant cotton;Viator;Weed Sci.,2004 5. Tolerance of transgenic cotton to topical applications of glyphosate;Jones;J. Cotton Sci.,1999
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