The Biochemical Response of Soybean Cultivars Infected by Diaporthe Species Complex
Author:
Petrović Kristina123ORCID, Šućur Elez Jovana4ORCID, Crnković Marina4, Krsmanović Slobodan15, Rajković Miloš16ORCID, Kuzmanović Boris4ORCID, Malenčić Đorđe4
Affiliation:
1. Institute of Field and Vegetable Crops, National Institute of the Republic of Serbia, 21000 Novi Sad, Serbia 2. Breeding Department, Maize Research Institute, 11185 Belgrade, Serbia 3. BioSense Institute, University of Novi Sad, 21101 Novi Sad, Serbia 4. Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Serbia 5. Sector for Plant Nutrition, Agromarket BiH, 76300 Bijeljina, Bosnia and Herzegovina 6. Department for Research and Development in Agriculture, Institute of Medicinal Plant Research “Dr. Josif Pančić”, 11000 Belgrade, Serbia
Abstract
Oxidative stress in soybean plants infected with Diaporthe isolates was evaluated in order to select (1) the least aggressive inoculation method, (2) to determine the most aggressive Diaporthe isolate, and (3) to determine the most tolerant soybean cultivar to this isolate. Based on the present malondialdehyde (MDA) content, the main end product of the lipid peroxidation process, and the biomarker for oxidative stress, the mycelium contact method was chosen as the least aggressive inoculation method, compared to the toothpick method and plug method. The activity of the antioxidant enzymes (superoxide–dismutase (SOD), catalase (CAT), and peroxidase (PX)), the reduced glutathione (GSH) content, and the level of lipid peroxidation (LP) were measured in soybean cv. Sava infected by five different Diaporthe species (DPM1F—D. aspalathi, DPC/KR19—D. caulivora, DPC004NY15—D. eres, 18-DIA-SOY-14—D. gulyae, and PL157A—D. longicolla). The most pathogenic Diaporthe species to cv. Sava was D. eres. The screening of the antioxidant enzymes activity in the leaves of 12 different soybean cultivars (Altona, Atlas, Capital, Chico, CX134, Favorit, Lakota, McCall, Morsoy, Strain, Rubin, and Victoria) infected with D. eres by the mycelium contact inoculation method showed that Capital, McCall, and Morsoy were the cultivars with the highest tolerance to D. eres, followed by Chico, Favorit, Lakota, and Rubin. The most sensitive cultivars were Atlas, CX134, Victoria, and Strain.
Funder
European Union’s Horizon 2020 Project—ECOBREED—Increasing the efficiency and competitiveness of organic crop breeding Ministry of Science, Technological Development and Innovation of the Republic of Serbia
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference36 articles.
1. Soybean;Gupta;Technological Innovations in Major World Oil Crops,2012 2. A review of soybean (Glycine max) seed, pod, and flower mycofloras in North America, with methods and a key for identification of selected fungi;Roy;Mycopathologia,2001 3. Hartman, G.L., Rupe, J.C., Sikora, E.J., Domier, L.L., Davies, J.A., and Steffey, K.L. (2015). Compendium of Soybean Diseases and Pests, The American Phytopathological Society. [5th ed.]. 4. Soybean stem canker caused by Diaporthe caulivora; Pathogen diversity, Colonization Process, and Plant Defense Activation;Mena;Front. Plant Sci.,2020 5. Petrović, K. (2012). Morphological, Molecular and Pathogenic Characterization of Species Diaporthe/Phomosis on Soybean in Serbia. [Ph.D. Thesis, Faculty of Agriculture, University of Belgrade].
|
|