Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb.

Author:

Ruiz Marta1,Vo Annie Du1,Becker J. Ole2ORCID,Roose Mikeal L.1ORCID

Affiliation:

1. Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA

2. Department of Nematology, University of California, Riverside, CA 92521, USA

Abstract

As pest management options, such as nematicides, become more restrictive, developing rootstocks resistant to the citrus nematode is fundamental for citrus production. This study provides an updated methodology to screen for citrus nematode resistance in rootstock-breeding programs. We developed a novel method to extract female citrus nematodes from roots that is suitable for molecular work and a real-time-PCR-based nematode quantification method for Tylenchulus semipenetrans. These procedures allow scaling up screening to high-throughput workflows, increasing the chances of finding rootstock candidates that combine all the desired traits. Our results contribute to the growing literature supporting quantification of nematodes with molecular methods.

Funder

California Citrus Research Board

Publisher

MDPI AG

Subject

Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics

Reference38 articles.

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2. Distribution and losses of Tylenchulus semipenetrans in citrus orchards on reclaimed land in Egypt;Koura;Nematology,2016

3. Sikora, R., Coyne, D., Hallmann, J., and Timper, P. (2018). Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, CABI. [3rd ed.].

4. Timmer, L.W., and Duncan, L.W. (1999). Citrus Health Management, American Phytopathological Society Press.

5. Population densities of Tylenchulus semipenetrans related to physicochemical properties of soil and yield of clementine mandarin in Spain;Sorribas;Plant Dis.,2008

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