Characterization of microRNAs and Target Genes in Musa acuminata subsp. burmannicoides, var. Calcutta 4 during Interaction with Pseudocercospora musae
Author:
Rego Erica Cristina Silva1, Pinheiro Tatiana David Miranda1, Fonseca Fernando Campos de Assis2ORCID, Gomes Taísa Godoy1, Costa Erica de Castro1ORCID, Bastos Lucas Santos1ORCID, Alves Gabriel Sergio Costa1, Cotta Michelle Guitton1, Amorim Edson Perito3ORCID, Ferreira Claudia Fortes3ORCID, Togawa Roberto Coiti4ORCID, Costa Marcos Mota Do Carmo4, Grynberg Priscila4ORCID, Miller Robert Neil Gerard1ORCID
Affiliation:
1. Instituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, DF, Brazil 2. Departament of Academic Areas, Instituto Federal de Goiás (IFG), Águas Lindas 72910-733, GO, Brazil 3. Embrapa Cassava and Tropical Fruits, Cruz das Almas 44380-000, BA, Brazil 4. Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, CP 02372, Brasília 70770-917, DF, Brazil
Abstract
Endogenous microRNAs (miRNAs) are small non-coding RNAs that perform post-transcriptional regulatory roles across diverse cellular processes, including defence responses to biotic stresses. Pseudocercospora musae, the causal agent of Sigatoka leaf spot disease in banana (Musa spp.), is an important fungal pathogen of the plant. Illumina HiSeq 2500 sequencing of small RNA libraries derived from leaf material in Musa acuminata subsp. burmannicoides, var. Calcutta 4 (resistant) after inoculation with fungal conidiospores and equivalent non-inoculated controls revealed 202 conserved miRNAs from 30 miR-families together with 24 predicted novel miRNAs. Conserved members included those from families miRNA156, miRNA166, miRNA171, miRNA396, miRNA167, miRNA172, miRNA160, miRNA164, miRNA168, miRNA159, miRNA169, miRNA393, miRNA535, miRNA482, miRNA2118, and miRNA397, all known to be involved in plant immune responses. Gene ontology (GO) analysis of gene targets indicated molecular activity terms related to defence responses that included nucleotide binding, oxidoreductase activity, and protein kinase activity. Biological process terms associated with defence included response to hormone and response to oxidative stress. DNA binding and transcription factor activity also indicated the involvement of miRNA target genes in the regulation of gene expression during defence responses. sRNA-seq expression data for miRNAs and RNAseq data for target genes were validated using stem-loop quantitative real-time PCR (qRT-PCR). For the 11 conserved miRNAs selected based on family abundance and known involvement in plant defence responses, the data revealed a frequent negative correlation of expression between miRNAs and target host genes. This examination provides novel information on miRNA-mediated host defence responses, applicable in genetic engineering for the control of Sigatoka leaf spot disease.
Funder
Fundação de Amparo à Pesquisa do Estado de Distrito Federal Instituto Nacional de Ciência e Tecnologia CAPES CNPq
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference125 articles.
1. The genetic structure of Australian populations of Mycosphaerella musicola suggests restricted gene flow at the continental scale;Hayden;Phytopathology,2005 2. Molecular diagnostics for the Sigatoka disease complex of banana;Arzanlou;Phytopathology,2007 3. Genetic diversity and azole fungicide sensitivity in Pseudocercospora musae field populations in Brazil;Brito;Front. Microbiol.,2020 4. Wang, Z., Zhang, J., Jia, C., Liu, J., Li, Y., Yin, X., Xu, B., and Jin, Z. (2012). De novo characterization of the banana root transcriptome and analysis of gene expression under Fusarium oxysporum f. sp. cubense tropical race 4 infection. BMC Genom., 13. 5. Li, C.-Y., Deng, G.-M., Yang, J., Viljoen, A., Jin, Y., Kuang, R.-B., Zuo, C.-W., Lv, Z.-C., Yang, Q.-S., and Sheng, O. (2012). Transcriptome profiling of resistant and susceptible Cavendish banana roots following inoculation with Fusarium oxysporum f. sp. cubense tropical race 4. BMC Genom., 13.
|
|