Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in Ilex paraguariensis Roots
Author:
Avico Edgardo H.1, Acevedo Raúl M.1ORCID, Duarte María J.1, Rodrigues Salvador Acácio2, Nunes-Nesi Adriano2, Ruiz Oscar A.3ORCID, Sansberro Pedro A.1ORCID
Affiliation:
1. Laboratorio de Biotecnología Aplicada y Genómica Funcional, Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto. Cabral 2131, Corrientes W3402BKG, Argentina 2. National Institute of Science and Technology on Plant Physiology under Stress Conditions, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa 36570-900, MG, Brazil 3. Unidad de Biotecnología 1, IIB-INTECH (UNSAM-CONICET), Chascomús B7130IWA, Argentina
Abstract
The appearance of water stress episodes triggers leaf abscission and decreases Ilex paraguariensis yield. To explore the mechanisms that allow it to overcome dehydration, we investigated how the root gene expression varied between water-stressed and non-stressed plants and how the modulation of gene expression was linked to metabolite composition and physiological status. After water deprivation, 5160 differentially expressed transcripts were obtained through RNA-seq. The functional enrichment of induced transcripts revealed significant transcriptional remodelling of stress-related perception, signalling, transcription, and metabolism. Simultaneously, the induction of the enzyme 9-cis-expoxycarotenoid dioxygenase (NCED) transcripts reflected the central role of the hormone abscisic acid in this response. Consequently, the total content of amino acids and soluble sugars increased, and that of starch decreased. Likewise, osmotic adjustment and radical growth were significantly promoted to preserve cell membranes and water uptake. This study provides a valuable resource for future research to understand the molecular adaptation of I. paraguariensis plants under drought conditions and facilitates the exploration of drought-tolerant candidate genes.
Funder
Research Council of Argentina Agencia Nacional de Promoción Científica y Técnica Universidad Nacional del Nordeste Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference115 articles.
1. Variability of vessel characteristics in the xylem of Ilex paraguariensis (maté-tree) from south Brazil;Reissmann;IAWA J.,2004 2. Gawron-Gzella, A., Chanaj-Kaczmarek, J., and Cielecka-Piontek, J. (2021). Yerba Mate—A Long but Current History. Nutrients, 13. 3. Pörtner, H.-O., Roberts, D.C., Tignor, M., Poloczanska, E.S., Mintenbeck, K., Alegría, A., Craig, M., Langsdorf, S., Löschke, S., and Möller, V. (2022). Contribution of Working Group II to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change, Cambridge University Press. 4. A succinate dehydrogenase flavoprotein subunit-like transcript is up-regulated in Ilex paraguariensis leaves in response to water deficit and abscisic acid;Acevedo;Plant Physiol. Biochem.,2013 5. Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves;Acevedo;Planta,2019
|
|