Cytotoxicity of Nine Medicinal Plants from San Basilio de Palenque (Colombia) on HepG2 Cells
Author:
Caballero-Gallardo Karina12ORCID, Alvarez-Ortega Neda12, Olivero-Verbel Jesus1ORCID
Affiliation:
1. Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, Colombia 2. Functional Toxicology Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, Colombia
Abstract
The utilization of plants with medicinal properties is deeply rooted in the traditional knowledge of diverse human populations. This study aims to investigate the cytotoxicity of nine plants commonly used by communities in San Basilio de Palenque, Bolivar (Colombia), for managing inflammation-related illnesses. Hydroethanolic extracts from various plant parts such as roots, stems, barks, or leaves were prepared through a process involving drying, powdering, and maceration in an ethanol–water (7:3) solution. The extracts were subsequently freeze-dried and dissolved in DMSO for the bioassays. Cytotoxicity against the human hepatoma HepG2 cell line was assessed using the MTT assay, with extract concentrations ranging from 0 to 500 µg/mL and treatment durations of 24 and 48 h. The total phenolic content of the nine extracts varied from 96.7 to 167.6 mg GAE/g DT. Among them, eight hydroethanolic extracts from Jatropha gossypiifolia L., Piper peltatum L., Malachra alceifolia, Verbesina turbacensis, Ricinus communis, Desmodium incanum, and Dolichandra unguis-cati showed low toxicity (IC50 > 500 µg/mL, 24 h) against HepG2 cells. On the other hand, the extracts of Aristolochia odoratissima L. (IC50 = 95.7 µg/mL) and Picramnia latifolia (IC50 = 128.9 µg/mL) demonstrated the highest cytotoxicity against the HepG2 cell line, displaying a modest selectivity index when compared to the HEKn cell line after 48 h of treatment. These findings suggest that medicinal plants from San Basilio de Palenque, particularly Picramnia latifolia and Aristolochia odoratissima, have potential activity against cancer cells, highlighting their potential for further research and development.
Funder
Ministry of Science, Technology and Innovation (Minciencias) and University of Cartagena the Ministry of Science, Technology and Innovation the Ministry of Education, the Ministry of Industry, Commerce and Tourism, and ICETEX Program Ecosistema Científico–Colombia Científica Francisco José de Caldas Fund
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference38 articles.
1. Worldwide importance of medicinal plants: Current and historical perspectives;Aslam;J. Recent Adv. Biol. Med.,2016 2. Shafi, A., Hassan, F., Zahoor, I., Majeed, U., and Khanday, F.A. (2021). Biodiversity, Management and Sustainable Use of Medicinal and Aromatic Plant Resources. Medicinal and Aromatic Plants, Springer. 3. Review on application and management of medicinal plants for the livelihood of the local community;Beyene;J. Resour. Dev,2016 4. Conservation and sustainable use of medicinal plants: Problems, progress, and prospects;Chen;J. Chin. Med.,2016 5. Hernández, J.A.C., and Andrea, J. (2011). Caracterización Fitoquímica, Actividad Antibacteriana y Antioxidante de Extractos de Plantas Medicinales Utilizadas en Pereira y Santa Rosa de Cabal (Risaralda), Tecnología Química. Universidad Tecnológica de Pereira, Facultad de Tecnologías.
|
|