The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency-Reference-Cited by-同舟云学术

The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency

Published:2023-07-27 Issue:15 Volume:12 Page:2782
ISSN:2223-7747
Container-title:Plants
language:en
Short-container-title:Plants

Author:

Bakirbas Ahmet¹^ORCID,Castro-Rodriguez Rosario²,Walker Elsbeth L.²^ORCID

Affiliation:

1. Biology Department and Plant Biology Graduate Program, University of Massachusetts, Amherst, MA 01003, USA

2. Biology Department, University of Massachusetts, Amherst, MA 01003, USA

Abstract

In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of Arabidopsis thaliana, and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size—less than 200 nt. Moreover, typical rRNA bands were not observed. Sequencing of eight independent phloem RNA samples indicated that tRNA-derived fragments, specifically 5′ tRFs and 5′ tRNA halves, are highly abundant in phloem sap, comprising about 46% of all reads. In addition, a set of miRNAs that are present in phloem sap was defined, and several miRNAs and sRNAs were identified that are differentially expressed during iron deficiency.

Funder

U.S. National Science Foundation

Publisher

MDPI AG

Subject

Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics

Link

https://www.mdpi.com/2223-7747/12/15/2782/pdf

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