Affiliation:
1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Abstract
Plant chitinases (EC 3.2.1.14) are pathogenesis-related (PR) proteins and are well studied in many plant species. However, little is known about the genomic organization and expression of chitinase genes in strawberries (Fragaria vesca). Here, 23 FvChi genes were identified in the genome of strawberry (F. vesca) and divided into GH18 and GH19 subfamilies based on phylogenetic relationships. A detailed bioinformatics analysis of the FvChi genes was performed, including gene physicochemical properties, chromosomal location, exon–intron distribution, domain arrangement, and subcellular localization. Twenty-two FvChi genes showed upregulation after Colletotrichum gloeosporioides infection. Following the exogenous application of SA, FvChi-3, 4, and 5 showed significant changes in expression. The ectopic expression of FvChi-14 in Arabidopsis thaliana increased resistance to C. higginsianum via controlling the SA and JA signaling pathway genes (AtPR1, AtICS1, AtPDF1.2, and AtLOX3). The FvChi-14 protein location was predicted in the cell wall or extracellular matrix. We speculate that FvChi-14 is involved in disease resistance by regulating the SA and JA signaling pathways. The findings of this study provide a theoretical reference for the functional studies of FvChi genes and new candidates for strawberry stress resistance breeding programs.
Funder
National Natural Science Foundation of China
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference59 articles.
1. Evolution and Expression Divergence of the Chitinase Gene Family against Leptosphaeria maculans and Sclerotinia sclerotiorum Infection in Brassica napus;Wen;J. Henan Agric. Sci.,2020
2. Lv, P., Zhang, C., Xie, P., Yang, X., El-Sheikh, M.A., Hefft, D.I., Ahmad, P., Zhao, T., and Bhat, J.A. (2022). Genome-Wide Identification and Expression Analyses of the Chitinase Gene Family in Response to White Mold and Drought Stress in Soybean (Glycine max). Life, 12.
3. Genome-wide Identification and Expression Analysis of CTL Gene Family Memebers in Capsicum annuum L.;Liu;Chin. J. Trop. Crops,2021
4. Identification of the Eucalyptus grandis chitinase gene family and expression characterization under different biotic stress challenges;Tobias;Tree Physiol.,2017
5. Genome-wide identification and expression profiling of chitinase genes in tea (Camellia sinensis (L.) O. Kuntze) under biotic stress conditions;Bordoloi;Physiol. Mol. Biol. Plants,2021
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献