Affiliation:
1. Floriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA), Wanju 55365, Republic of Korea
Abstract
Rose (Rosa hybrida) is a major flower crop worldwide and has long been loved for its variety of colors and scents. Roses are mainly used for gardening or cutting flowers and are also used as raw materials for perfumes, cosmetics, and food. Essential oils, which are extracted from the flowers of plants, including roses, have various scents, and the essential oil market has been growing steadily owing to the growing awareness of the benefits of natural and organic products. Therefore, it is necessary to develop a system that stably supplies raw materials with uniform ingredients in line with the continuous increase in demand. In this study, conditions for the efficient induction of callus were established from the petals of the rose breeding line 15R-12-2, which has a strong scent developed by the National Institute of Horticultural and Herbal Science, Rural Development Administration. The highest callus induction rate (65%) was observed when the petals of the fully open flower (FOF) were placed on the SH11DP medium so that the abaxial surface was in contact with the medium. In addition, the VOCs contained in the petals of 15R-12-2 and the petal-derived callus were analyzed by HS-SPME-GC-MS. Thirty components, including esters and alcohols, were detected in the petal-derived callus. Among them, 2-ethylhexan-1-ol, which showed 59.01% relative content when extracted with hexane as a solvent, was the same component as detected in petals. Therefore, petal-derived callus is expected to be of high industrial value and can be suggested as an alternative pathway to obtaining VOCs.
Funder
Rural Development Administration of Korea
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference49 articles.
1. Essential oils: Brief background and uses;Naeem;Ann. Short Rep.,2018
2. Flament, I., Debonneville, C., and Furrer, A. (1993). Bioactive Volatile Compounds from Plants, American Chemical Society.
3. Biochemical and molecular genetic aspects of floral scents;Dudareva;Plant Physiol.,2000
4. Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/citronellol acetyltransferase in developing rose petals;Shalit;Plant Physiol.,2003
5. Both the adaxial and abaxial epidermal layers of the rose petal emit volatile scent compounds;Bergougnoux;Planta,2007