Antitumor Effect of Epigallocatechin Gallate and Vincristine in Mice with L5178Y Lymphoma
Author:
Almaguer Georgina1ORCID, Almaguer-Vargas Gustavo2ORCID, Molina-Trinidad Eva María1ORCID, Becerril-Flores Marco Antonio1ORCID, Montejano Brenda3ORCID, Madrigal-Santillan Eduardo1ORCID, Hernández-Ceruelos Alejandra1ORCID, Figueroa-Gutiérrez Ana Hilda1ORCID, Montejano Ethoan4ORCID, Montejano-Rodríguez José Ramón1ORCID
Affiliation:
1. Institute of Health Sciences, Autonomous University of the State of Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla 42183, Mexico 2. Plant Breeding Department, Horticulture Institute, Chapingo Autonomous University, Federal Highway Mexico-Texcoco km 38.5, Chapingo, Texcoco 56230, Mexico 3. Department of Pharmacy, Hospital San José, Santiago de Querétaro 76180, Mexico 4. Interdisciplinary Professional Unit of Biotechnology IPN, National Polytechnic Institute, Av. Acueducto, La Laguna Ticoman, Gustavo A. Madero, Mexico City 07340, Mexico
Abstract
The main objective of research into new therapies is the search for more efficacy and fewer toxic effects in cancer treatments. On one hand, vincristine (VCR) is a chemotherapeutic used in different kinds of tumors. On the other hand, epigallocatechin gallate (EGCG) is a green tea metabolite that has shown an antineoplastic effect in diverse investigations, so the objective of this work is to evaluate the antitumor effects of the EGCG/VCR combination on tumor volume and survival. To achieve this objective, the solid model of lymphoma L5178Y was used in BALB/c mice with different doses of VCR, EGCG, and their combination allowed tumor growth and survival time recording. After tumor collection, measurements, and immunohistochemistry for p53, Bcl2, and Cyclin D1 were performed. The results showed that the EGCG/vincristine combination had a greater antitumor effect than those effects of vincristine and EGCG. It can be attributed to the fact that the greatest inhibition of Bcl2 was present in gathering of EGCG harvest with vincristine. Therefore, the combination of EGCG with vincristine has a better antineoplastic effect by inhibiting tumor development and increasing survival on both substances independently.
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
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