Toxicity and Anti-Inflammatory Effects of Agave sisalana Extract Derived from Agroindustrial Residue

Author:

Costa Luisa Taynara Silvério da12ORCID,Fracasso Julia Amanda Rodrigues12,Guarnier Lucas Pires3,Brito Gustavo Reis de4ORCID,Fumis Daniel Baldini5,Camargo Bittencourt Renata Aparecida de6,Guiotti Aimée Maria7,Barros Barbosa Débora de7ORCID,Camargo Isabel Cristina Cherici1,Souza Edislane Barreiros de1,Oliva Neto Pedro de1,Santos Lucinéia dos12

Affiliation:

1. Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, SP, Brazil

2. School of Dentistry, São Paulo State University (UNESP), Araçatuba 05508-000, SP, Brazil

3. Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14049-900, SP, Brazil

4. Department of Biology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, SP, Brazil

5. Departament of Materials Science and Technology, Bauru School of Science, São Paulo State University (UNESP), Bauru 17033-360, SP, Brazil

6. Department of Biomedicine, Institute of Health Sciences, University of São Paulo (UNIP), Assis 19813-550, SP, Brazil

7. Department of Dental Materials and Prosthodontics, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16066-840, SP, Brazil

Abstract

Background: In several countries, the leaf juice of Agave sisalana (also known as sisal) is widely used topically, especially as an antiseptic, and orally for the treatment of different pathologies. However, in Brazil, which is the largest producer of Agave sisalana, its residue, which represents the majority of its weight, has been thrown away. For this reason, the determination of the pharmacological and toxicological potentials of sisal residue and its possible therapeutic use is seen as a way to contribute to the sustainable development and social promotion of the largest producer of sisal in Brazil, the interior of Bahia State, which is among the poorest areas in the country. Given the scarcity of available scientific studies on the pharmacological and toxicological properties of sisal residue juice, this study aimed to promote the acid hydrolysis of this juice to potentiate the anti-inflammatory effect already described in the literature. Furthermore, it aimed to evaluate the toxicological profile of the hydrolyzed extract (EAH) and to determine its acute toxicity, as well as its side effects on the reproductive aspects of rats. Method: The anti-inflammatory effect of EAH was evaluated in vitro using the induction of hemolysis by hypotonic solution and in vivo in rats using the carrageenan-induced paw edema test and the xylene-induced ear edema test. The acute toxicity, resulting from a single-dose administration, was investigated for some manifestation of toxic symptoms related to motor control and consciousness in rats. At a concentration of 100 mg/kg, by repeated doses, the reproductive toxicity effects of EAH in rats were assessed. Results: In vitro anti-inflammatory activity was positive using the human red blood cell membrane stabilization method. In both in vivo tests used to assess the anti-inflammatory activity, EAH (at three doses) significantly inhibited edema when compared to the control group. At a dose of 50 mg/kg, EAH exhibited a greater effect than indomethacin, a nonsteroidal anti-inflammatory drug with known activity. In vivo toxicological studies have shown that EAH does not present toxic effects when administered orally in a single dose, up to 1000 mg/kg. Finally, EAH promoted a gonadotoxic effect and increased the embryonic mortality rate after implantation. Conclusions: It is suggested that the anti-edematogenic effect of the acid hydrolysis extract from sisal juice is due to the high concentration of steroidal sapogenins. Therefore, this extract can be considered a potential new anti-inflammatory or even an important sapogenin source for the development of steroidal glucocorticoids. However, further studies are needed to elucidate the chemical composition of sisal juice. Regarding toxicology studies, EAH did not show cytotoxic and clastogenic potentials, but it presented a powerful reproductive toxic effect in rats.

Funder

FAPESP

CNPq

Publisher

MDPI AG

Subject

Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics

Reference33 articles.

1. Instituto Brasileiro De Geografia E Estatística (IBGE) (2021, December 08). Sistema IBGE de Recuperação Automática, Available online: https://sidra.ibge.gov.br/Tabela/1613.

2. Endophytic Bacteria Isolated from Both Healthy and Diseased Agave sisalana Plants Are Able to Control the Bole Rot Disease;Silva;Biol. Control,2021

3. Experimental Investigation on Self Compacting Concrete using Sisal Fibre;Omkar;IJESC,2019

4. Jawaid, M., Thariq, M., and Saba, N. (2019). Mechanical and Physical Testing of Biocomposites, Fibre-Reinforced Composites and Hybrid Composites, Woodhead Publishing.

5. Sisal and its Potential for Creating Innovative Employment Opportunities and Economic Prospects;Srinivasakumar;IOSR J. Mech. Civ. Eng.,2013

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3