Measurement of Adult Human Brain Responses to Breath-Holding by Multi-Distance Hyperspectral Near-Infrared Spectroscopy

Abstract

A major limitation of near-infrared spectroscopy (NIRS) is its high sensitivity to the scalp and low sensitivity to the brain of adult humans. In the present work we used multi-distance hyperspectral NIRS (hNIRS) to investigate the optimal source-detector distances, wavelength ranges, and analysis techniques to separate cerebral responses to 30 s breath-holds (BHs) from the responses in the superficial tissue layer in healthy adult humans. We observed significant responses to BHs in the scalp hemodynamics. Cerebral responses to BHs were detected in the cytochrome C oxidase redox (rCCO) at 4 cm without using data from the short-distance channel. Using the data from the 1 cm channel in the two-layer regression algorithm showed that cerebral hemodynamic and rCCO responses also occurred at 3 cm. We found that the waveband 700–900 nm was optimal for the detection of cerebral responses to BHs in adults.