The Response of Arbuscular Mycorrhizal Fungal Communities to the Soil Environment of Underground Mining Subsidence Area in Northwest China

Abstract

Fully mechanized mining technology applied over a very large working face is typically utilized for coal exploitation in Northwest China and triggered two types of land subsidence above the goaf edge and center. However, the effects of mining subsidence on arbuscular mycorrhizal fungal (AMF) communities are still unknown. Here, we investigated the soil physicochemical properties and the response of AMF communities to the soil environment at the margin and center of the subsidence area of the same working face. Our results showed the soil water content, nutrient content and enzyme activity were significantly decreased with land desertification at the margin of the subsidence area but were less affected at the subsidence center. Utilizing high-throughput sequence analysis, six Glomeromycotan genera were detected. The relative abundance of Glomus and Ambispora at the margin of the subsidence area decreased, while Paraglomus and Diversispora increased. The total OTU richness was significantly correlated with moisture. Redundancy analysis showed the main environmental factors driving the changes in AMF community structure were available nitrogen, available potassium and available phosphorus. All these results indicated land cracks need to be repaired in time at subsidence edge to prevent the decline of soil fertility.