Characterization of Green Fluorescent Protein in Heart Valves of a Transgenic Swine Model for Partial Heart Transplant Research-Reference-Cited by-同舟云学术

Characterization of Green Fluorescent Protein in Heart Valves of a Transgenic Swine Model for Partial Heart Transplant Research

Published:2023-06-10 Issue:6 Volume:10 Page:254
ISSN:2308-3425
Container-title:Journal of Cardiovascular Development and Disease
language:en
Short-container-title:JCDD

Author:

Bishara Katherine¹^ORCID,Kwon Jennie H.¹,Hill Morgan A.¹,Helke Kristi L.²,Norris Russell A.³,Whitworth Kristin⁴,Prather Randall S.⁴^ORCID,Rajab Taufiek Konrad¹

Affiliation:

1. Department of Surgery, Medical University of South Carolina, Charleston, SC 29501, USA

2. Department of Comparative Medicine, Medical University of South Carolina, Charleston, SC 29501, USA

3. Department of Regenerative Medicine, Medical University of South Carolina, Charleston, SC 29501, USA

4. National Swine Resource and Research Center, University of Missouri, Columbia, MO 65211, USA

Abstract

A transgenic strain of pigs was created to express green fluorescent protein (GFP) ubiquitously using a pCAGG promoter. Here, we characterize GFP expression in the semilunar valves and great arteries of GFP-transgenic (GFP-Tg) pigs. Immunofluorescence was performed to visualize and quantify GFP expression and colocalization with nuclear staining. GFP expression was confirmed in both the semilunar valves and great arteries of GFP-Tg pigs compared to wild-type tissues (aorta, p = 0.0002; pulmonary artery, p = 0.0005; aortic valve; and pulmonic valve, p < 0.0001). The quantification of GFP expression in cardiac tissue allows this strain of GFP-Tg pigs to be used for future research in partial heart transplantation.

Funder

National Heart, Lung, And Blood Institute of the National Institutes of Health

Director of the national Institutes of Health

Publisher

MDPI AG

Subject

Pharmacology (medical),General Pharmacology, Toxicology and Pharmaceutics

Link

https://www.mdpi.com/2308-3425/10/6/254/pdf

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