Abstract
Nineteen microsatellite loci, obtained by the whole genome sequencing approach, were developed and validated for the ‘smooth cauliflower’ coral Stylophora pistillata, a widespread Indo Pacific branching coral species. A sample size of 40 colonies collected at five reef sites along the northern Gulf of Eilat, the Red Sea, were genotyped, revealing loci reproducibly and suitable outcomes for wide applications, including population genetic studies. The 19 new microsatellite loci in this sample were composed of 4–20 alleles/locus, of which 10 microsatellites are highly polymorphic (≥10 alleles/locus). The observed and expected heterozygosity ranged between 0.289 and 0.957 (mean 0.597) and 0.101 and 0.911 (mean 0.726), respectively, and the Fixation Index (F), which also indicates the inbreeding coefficient, ranges between −0.174 and 0.569 (mean 0.207). The polymorphic information content (PIC) ranges between 0.100 and 0.904 (mean 0.699). This new set of microsatellite loci will be employed for population genetics studies as for identifying the distribution of various genotypes within S. pistillata chimeras.
Subject
Ocean Engineering,Water Science and Technology,Civil and Structural Engineering
Reference30 articles.
1. Shefy, D., and Rinkevich, B. (2021). Handbook of Marine Model Organisms in Experimental Biology, CRC Press.
2. The Reproduction of the Red Sea Coral Stylophora Pistillata from Eilat: 4-Decade Perspective;Shefy;Mar. Biol.,2018
3. Genetic Variation of the Scleractinian Coral Stylophora Pistillata, from Western Pacific Reefs;Takabayashi;Coral Reefs,2003
4. Genotypic Diversity and Gene Flow in Brooding and Spawning Corals along the Great Barrier Reef, Australia;Ayre;Evolution,2000
5. Local Scale Genetic Structure in Coral Populations: A Signature of Selection;Zvuloni;Mar. Pollut. Bull.,2008