Differential Expression of microRNAs in Hypertrophied Myocardium and Their Relationship to Late Gadolinium Enhancement, Left Ventricular Hypertrophy and Remodeling in Hypertrophic Cardiomyopathy

Abstract

Background: Differential expression has been found in a variety of circulating miRNAs in patients with hypertrophic cardiomyopathy (HCM). However, study on myocardial miRNAs is limited and a lot of miRNAs were not studied in previous studies. Methods: Twenty-one HCM patients and four patients who died from non-cardiovascular diseases were prospectively recruited for our study. A total of 26 myocardial tissues were collected, which were stored in liquid nitrogen immediately for miRNA detection using the Agilent Human miRNA Microarray Kit. All HCM patients underwent cardiovascular magnetic resonance (CMR) examination before surgery and cvi42 software was used to analyze cardiac function and myocardial fibrosis. Results: Compared with the control group, the expression of 22 miRNAs was found to be significantly increased in the HCM group, while 46 miRNAs were found to be significantly decreased in the HCM group. The expression levels of hsa-miR-3960 and hsa-miR-652-3p were significantly correlated with left ventricular mass index (r = 0.449 and 0.474, respectively). Meanwhile, Hsa-miR-642a-3p expression was positively correlated to the quantification of late gadolinium enhancement (r = 0.467). Conclusions: Our study found that 68 myocardial miRNAs were significantly increased or decreased in the HCM group. Myocardial miRNA levels could be used as potential biomarkers for LV hypertrophy, fibrosis and remodeling.