Enterobacterales Biofilm-Specific Genes and Antimicrobial and Anti-Inflammatory Biomarkers in the Blood of Patients with Ischemic Heart Disease

Author:

Giedraitiene Agne1,Tatarunas Vacis2,Kaminskaite Kornelija3,Meskauskaite Ugne3,Boieva Svitlana2,Ajima Yu34,Ciapiene Ieva2ORCID,Veikutiene Audrone2,Zvikas Vaidotas5,Kupstyte-Kristapone Nora3,Jakstas Valdas5,Luksiene Dalia2ORCID,Tamosiunas Abdonas2ORCID,Lesauskaite Vaiva2

Affiliation:

1. Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Eiveniu 4, LT 50161 Kaunas, Lithuania

2. Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu 15, LT 50103 Kaunas, Lithuania

3. Medical Academy, Lithuanian University of Health Sciences, A. Mickeviciaus 9, LT 44307 Kaunas, Lithuania

4. School of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan

5. Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu 13, LT 50161 Kaunas, Lithuania

Abstract

Background: Ischemic heart disease (IHD) is the most prevalent type of cardiovascular disease. The main cause of IHD is atherosclerosis, which is a multifactorial inflammatory disease of blood vessels. Studies show that bacteria might have a significant impact on the pathogenesis of atherosclerosis and plaque rupture. This study aimed to evaluate the complexity of interactions between bacteria and the human body concerning metabolites and bacterial genes in patients with ischemic heart disease. Methods: Bacterial 16S rDNA and wcaF, papC, and sdhC genes were detected in whole blood using a real-time PCR methodology. An enzyme-linked immunosorbent assay was used to measure the concentration of the LL-37 protein. An analysis of ARA in blood plasma was performed. Results: Bacterial 16S rDNA was detected in 31% of the study patients, and the genes wcaF and sdhC in 20%. Enterobacterales genes were detected more frequently in patients younger than 65 years than in patients aged 65 years and older (p = 0.018) and in patients with type 2 diabetes (p = 0.048). Concentrations of the human antimicrobial peptide LL-37 and 12S-HETE concentrations were determined to be higher if patients had 16S rDNA and biofilm-specific genes. Conclusions: The results of this study enhance the understanding that Enterobacterales bacteria may participate in the pathogenesis of atherosclerosis and IHD. Bacterial DNA and host metabolites in higher concentrations appear to be detected.

Funder

Medical Academy

Publisher

MDPI AG

Reference66 articles.

1. Global Epidemiology of Ischemic Heart Disease: Results from the Global Burden of Disease Study;Khan;Cureus,2020

2. (2022, March 15). European Cardiovascular Disease Statistics. Available online: https://ehnheart.org/cvd-statistics/cvd-statistics-2017.html.

3. (2022, April 23). An Official Website of the European Union. Available online: https://ec.europa.eu/eurostat/web/products-eurostat-news/-/edn-20200928-1.

4. Gu, D., and Dupre, M. (2020). Encyclopedia of Gerontology and Population Aging, Springer.

5. Pahwa, R., and Jialal, I. (2022). StatPearls, StatPearls Publishing. Available online: https://www.ncbi.nlm.nih.gov/books/NBK507799/.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3