Assessment of Cross-Reactivity of Chimeric Trypanosoma cruzi Antigens with Crithidia sp. LVH-60A: Implications for Accurate Diagnostics-Reference-Cited by-同舟云学术

Assessment of Cross-Reactivity of Chimeric Trypanosoma cruzi Antigens with Crithidia sp. LVH-60A: Implications for Accurate Diagnostics

Published:2023-11-17 Issue:22 Volume:13 Page:3470
ISSN:2075-4418
Container-title:Diagnostics
language:en
Short-container-title:Diagnostics

Author:

Santos Emily F.¹,Daltro Ramona T.²,Regis-Silva Carlos G.¹²,Pavan Tycha B. S.¹^ORCID,de Oliveira Fabrícia A.³,da Silva Ângela M.³⁴,Almeida Roque P.³⁴,Gonçalves Noilson L. S.¹,Sampaio Daniel D.⁵,Santos Faber N.⁶,Marchini Fabricio K.⁷⁸,Celedon Paola A. F.⁹,Zanchin Nilson I. T.²¹⁰,Santos Fred L. N.¹²^ORCID

Affiliation:

1. Advanced Public Health Laboratory, Gonçalo Moniz Institute, Oswaldo Cruz Foundation (Fiocruz-BA), Salvador 40296-710, BA, Brazil

2. Integrated Translational Program in Chagas Disease from Fiocruz (Fio-Chagas), Oswaldo Cruz Foundation (Fiocruz-RJ), Rio de Janeiro 21040-360, RJ, Brazil

3. Immunology and Molecular Biology Laboratory, University Hospital/EBSERH, Federal University of Sergipe, Aracaju 49060-676, SE, Brazil

4. Department of Medicine, University Hospital (BSERH), Federal University of Sergipe, Aracaju 49060-676, SE, Brazil

5. Brazil’s Family Health Strategy, Municipal Health Department, Tremedal City Hall, Tremedal 45170-000, BA, Brazil

6. Faculty of Medicine, Santo Agostinho College (FASA), Education Technology Healthcare, Vitória da Conquista 45028-100, BA, Brazil

7. Molecular Biology Institute of Paraná, Curitiba 81350-010, PR, Brazil

8. Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute (ICC), Oswaldo Cruz Foundation (Fiocruz-PR), Curitiba 81350-010, PR, Brazil

9. Laboratory of Molecular and Systems Biology of Trypanosomatids, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz-PR), Curitiba 81350-010, PR, Brazil

10. Structural Biology and Protein Engineering Laboratory, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz-PR), Curitiba 81350-010, PR, Brazil

Abstract

This study focuses on developing accurate immunoassays for diagnosing Chagas disease (CD), a challenging task due to antigenic similarities between Trypanosoma cruzi and other parasites, leading to cross-reactivity. To address this challenge, chimeric recombinant T. cruzi antigens (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) were synthesized to enhance specificity and reduce cross-reactivity in tests. While these antigens showed minimal cross-reactivity with leishmaniasis, their performance with other trypanosomatid infections was unclear. This study aimed to assess the diagnostic potential of these IBMP antigens for detecting CD in patients with Crithidia sp. LVH-60A, a parasite linked to visceral leishmaniasis-like symptoms in Brazil. This study involved seven Crithidia sp. LVH-60A patients and three Leishmania infantum patients. The results indicated that these IBMP antigens displayed 100% sensitivity, with specificity ranging from 87.5% to 100%, and accuracy values between 90% and 100%. No cross-reactivity was observed with Crithidia sp. LVH-60A, and only one L. infantum-positive sample showed limited cross-reactivity with IBMP-8.1. This study suggests that IBMP antigens offer promising diagnostic performance, with minimal cross-reactivity in regions where T. cruzi and other trypanosomatids are prevalent. However, further research with a larger number of Crithidia sp. LVH-60A-positive samples is needed to comprehensively evaluate antigen cross-reactivity.

Funder

Coordination for the Improvement of Higher Education Personnel in Brazil

Research Support Foundation of the State of Bahia

National Council for Scientific and Technological Development-Brazil

Publisher

MDPI AG

Subject

Clinical Biochemistry

Link

https://www.mdpi.com/2075-4418/13/22/3470/pdf

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