7α and 7β Hydroxylation of Dehydroepiandrosterone by Gibberella sp. and Absidia Coerulea Biotransformation-Reference-Cited by-同舟云学术

7α and 7β Hydroxylation of Dehydroepiandrosterone by Gibberella sp. and Absidia Coerulea Biotransformation

Published:2023-01-25 Issue:2 Volume:13 Page:272
ISSN:2073-4344
Container-title:Catalysts
language:en
Short-container-title:Catalysts

Author:

Song Ming¹,Fu Ruicheng²,Cai Sulan¹,Jiang Xuliang³^ORCID,Wang Fuju⁴,Xu Weizhuo²^ORCID,Xu Wei²

Affiliation:

1. School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China

2. School of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang 110016, China

3. School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China

4. Beijing Global Biotechnologies, Co., Ltd., Beijing 100193, China

Abstract

The hydroxylation of dehydroepiandrosterone (DHEA) to 7α -hydroxy-5-androstene-17-one (7α-OH-DHEA) and 7β-hydroxy-5-androstene-17-one (7β-OH-DHEA) by Gibberella sp. CICC 2498 and Absidia coerulea CICC 41050 was investigated. The media ingredients were optimized. Single factors such as the DHEA concentration, culture time, medium volume, and inoculum rate were individually investigated to generate optimum biotransformation conditions. An orthogonal optimization process using a four-factor, three- level L9 (33) experiment was designed and performed. Finally, the maximum production of 7β-OH-DHEA from DHEA biotransformation by Absidia coerulea is 69.61%. This strategy would provide a possible way to enhance the 7β-OH-DHEA yield in the pharmaceutical industry.

Publisher

MDPI AG

Subject

Physical and Theoretical Chemistry,Catalysis,General Environmental Science

Link

https://www.mdpi.com/2073-4344/13/2/272/pdf

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