Scale-Up Lipase Production and Development of Methanol Tolerant Whole-Cell Biocatalyst from Magnusiomyces spicifer SPB2 in Stirred-Tank Bioreactor and Its Application for Biodiesel Production
-
Published:2023-03-20
Issue:3
Volume:13
Page:617
-
ISSN:2073-4344
-
Container-title:Catalysts
-
language:en
-
Short-container-title:Catalysts
Author:
Srimhan Purimprat1,
Hongpattarakere Tipparat1
Affiliation:
1. Biotechnology Program, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand
Abstract
This study aimed to economically develop the yeast whole-cell biocatalyst from the lipase-secreting Magnusiomyces spicifer SPB2 to serve green biodiesel production. The scaled-up productions of lipases were optimized using a 5-L stirred-tank bioreactor. The maximum extracellular lipase and cell-bound lipase (CBL) yields of 1189.65 U/L and 5603.74 U/L were achieved at 24 h and 60 h, respectively, in the modified IMY medium (pH 5.0) containing 2% of soybean oil as a carbon source and 0.2% Gum Arabic as an emulsifying agent. The optimized cultivation was initiated with an inoculum size of 1 × 107 cells/mL and conducted under an aeration rate of 0.75 vvm with an agitation speed of 400 rpm. The obtained whole-cell biocatalyst of M. spicifer SPB2 was applied to catalyze the transesterification reaction using palm oil and methanol as substrates. The greatest yield of 97.93% fatty acid methyl ester (FAME) was reached at 72 h using a palm oil/methanol ratio of 1:7, indicating high methanol stability of the biocatalyst. Moreover, substrate homogenization accelerated the reaction to achieve FAME production of 97.01% at 48 h and remained stable afterwards. Without homogenization, the highest FAME of 98.20% was obtained at 60 h. The whole-cell biocatalyst prepared from lipase-secreting M. spicifer SPB2 at an up-scaled level greatly enhanced efficiency and feasibility for commercial biodiesel production through a green conversion process.
Funder
Higher Education Research Promotion
the Office of the Higher Education Commission and Annual Government of Expenditures
Subject
Physical and Theoretical Chemistry,Catalysis,General Environmental Science
Reference43 articles.
1. Lipases for biotechnology;Jaeger;Curr. Opin. Biotechnol.,2002
2. Recent advances on sources and industrial applications of lipases;Sarmah;Biotechnol. Prog.,2018
3. A valuable product of microbial cell factories: Microbial lipase;Yao;Front. Microbiol.,2021
4. Study of lipase production by Yarrowia lipolytica grown in high concentration of hydrophobic carbon sources;Colacicco;Chem. Eng. Trans.,2022
5. Influence of oxygen transfer on lipase production by Rhizopus arrhizus;Elibol;Process Biochem.,2000