Abstract
Despite all the psychological advantages of alprazolam, its long list of toxic properties and interactions has caused concern and highlighted the need for a reliable sensing method. In this study, we developed a simple, highly sensitive electrochemical nanobiosensor to determine the desirable dose of alprazolam, averting the undesirable consequences of overdose. Gold nanourchins (AuNUs) and iron-nickel reduced graphene oxide (Fe-Ni@rGO) were immobilized on a glassy carbon electrode, which was treated beforehand. The electrode surface was characterized using cyclic voltammetry, Fourier transform infrared spectroscopy, scanning electron microscopy/energy-dispersive X-ray spectroscopy, and differential pulse voltammetry. The fabricated sensor showed two linear ranges (4 to 500 µg L−1 and 1 to 50 mg L−1), low limit of detection (1 µg L−1), high sensitivity, good repeatability, and good recovery. Increased –OH and carboxyl (-COOH) groups on the electrode surface, resulting in improved the adsorption of alprazolam and thus lower limit of detection. This nanobiosensor could detect alprazolam powder dissolved in diluted blood serum; we also studied other benzodiazepine drugs (clonazepam, oxazepam, and diazepam) with this nanobiosensor, and results were sensible, with a significant difference.
Subject
Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)
Reference58 articles.
1. Benzodiazepine pharmacology and central nervous system–mediated effects;Griffin;Ochsner J.,2013
2. The history of benzodiazepines;Wick;Consult. Pharm.,2013
3. Usage of benzodiazepines: A review;Donoghue;Int. J. Psychiatry Clin. Pract.,2010
4. A Review of Alprazolam Use, Misuse, and Withdrawal;Ait-Daoud;J. Addict. Med.,2017
5. Benzodiazepine Misuse: An Epidemic Within a Pandemic;Sarangi;Cureus,2021
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