Effects of Different Feed Additives on Intestinal Metabolite Composition of Weaned Piglets
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Published:2024-02-26
Issue:3
Volume:14
Page:138
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ISSN:2218-1989
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Container-title:Metabolites
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language:en
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Short-container-title:Metabolites
Author:
Zhao Mingxuan123, Zhang Jian123, Liu Fuzhou123, Luo Lv123, Wei Mingbang123, Ye Yourong123, Yangzom Chamba123, Shang Peng123
Affiliation:
1. College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi 860000, China 2. The Key Laboratory of Tibetan Pigs Genetic Improvement and Reproduction Engineering, Linzhi 860000, China 3. The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi 860000, China
Abstract
To study the effects of different feed additives on the weaning stress of Tibetan piglets, we selected 28 healthy, 30-day-old Tibetan weaned piglets and divided them into four groups, namely, the control group (basal feed without any antibiotic additions) (Nor), the group with the addition of the antibiotic lincomycin (Ant), the group with the addition of fifteen-flavor black pills of Tibetan medicine (Tib), and the group with the addition of fecal bacterial supernatant (Fec). We measured growth performance, blood physiological indexes, and metabolomics. The results showed that the Ant, Tib, and Fec groups significantly reduced the ratio of diarrhea to feed/weight (F/G) and increased the average daily gain (ADG) compared with the Nor group (p < 0.01). The Nor group had significantly lower leukocyte counts, hemoglobin levels, and erythrocyte counts compared with the other three groups at 21 d (p < 0.05). These physiological indexes tended to stabilize at 42 d. We found that there were beneficial metabolites and metabolic pathways for gastrointestinal function. Specifically, the porphyrin metabolic pathway was elevated in the Ant group, and the tryptophan metabolic pathway was significantly elevated in the Tib and Fec groups compared with the Nor group (p < 0.05). In conclusion, adding fecal bacterial supernatant and fifteen-flavor black pills of Tibetan medicine to the feed reduced the rate of diarrhea and improved the growth performance of the piglets. Moreover, it had an effect on the microorganisms and their metabolites and pathways in the gastrointestinal tract of the animals, which might be the main reason for influencing the diarrhea rate of weaned Tibetan piglets and the growth and development of the piglets. This study provides a new approach for anti-stress applications in weaned Tibetan piglets and the development of substitute anti-products.
Funder
National Natural Fund Regional Fund Project national key research and development projects Science and Technology Program Project of the Tibet Autonomous Region
Reference55 articles.
1. Campbell, J.M., Crenshaw, J.D., and Polo, J. (2013). The biological stress of early weaned piglets. J. Anim. Sci. Biotechnol., 4. 2. Effect of very low-protein diets supplemented with branched-chain amino acids on energy balance, plasma metabolomics and fecal microbiome of pigs;Spring;Sci. Rep.,2020 3. Canibe, N., Hojberg, O., Kongsted, H., Vodolazska, D., Lauridsen, C., Nielsen, T.S., and Schönherz, A.A. (2022). Review on Preventive Measures to Reduce Post-Weaning Diarrhoea in Piglets. Animals, 12. 4. Lin, C., Wan, J., Su, Y., and Zhu, W. (2018). Effects of Early Intervention with Maternal Fecal Microbiota and Antibiotics on the Gut Microbiota and Metabolite Profiles of Piglets. Metabolites, 8. 5. Fecal microbiota transplant for relapsing Clostridium difficile infection using a frozen inoculum from unrelated donors: A randomized, open-label, controlled pilot study;Youngster;Clin. Infect. Dis.,2014
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