Metabolite Profiling Analysis of the Tongmai Sini Decoction in Rats after Oral Administration through UHPLC-Q-Exactive-MS/MS

Author:

Zheng Xianhui12,Zhan Yingying1,Peng Mengling1,Xu Wen12,Deng Guanghai12

Affiliation:

1. The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

2. Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China

Abstract

Tongmai Sini decoction (TSD), the classical prescriptions of traditional Chinese medicine, consisting of three commonly used herbal medicines, has been widely applied for the treatment of myocardial infarction and heart failure. However, the absorbed components and their metabolism in vivo of TSD still remain unknown. In this study, a reliable and effective method using ultra-performance liquid chromatography coupled with hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Exactive-MS/MS) was employed to identify prototype components and metabolites in vivo (rat plasma and urine). Combined with mass defect filtering (MDF), dynamic background subtraction (DBS), and neutral loss filtering (NLF) data-mining tools, a total of thirty-two major compounds were selected and investigated for their metabolism in vivo. As a result, a total of 82 prototype compounds were identified or tentatively characterized in vivo, including 41 alkaloids, 35 phenolic compounds, 6 saponins. Meanwhile, A total of 65 metabolites (40 alkaloids and 25 phenolic compounds) were tentatively identified. The metabolic reactions were mainly hydrogenation, demethylation, hydroxylation, hydration, methylation, deoxylation, and sulfation. These findings will be beneficial for an in-depth understanding of the pharmacological mechanism and pharmacodynamic substance basis of TSD.

Funder

Guangdong Provincial Hospital of Chinese Medicine

Scientific Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine

Publisher

MDPI AG

Reference45 articles.

1. Glycyrrhizae Radix et Rhizoma: The popular occurrence of herbal medicine applied in classical prescriptions;He;Phytother. Res.,2023

2. A comprehensive review of the classical prescription Yiguan Jian: Phytochemistry, quality control, clinical applications, pharmacology, and safety profile;Lu;J. Ethnopharmacol.,2024

3. Opportunities and challenges in development of compound preparations of traditional Chinese medicine: Problems and countermeasures in research of ancient classical prescriptions;Zhang;China J. Chin. Mater. Medica,2019

4. Comparative network pharmacology analysis of classical TCM prescriptions for chronic liver disease;Chen;Front. Pharmacol.,2019

5. Pharmacological research progress of five classical prescriptions in treatment of chronic heart failure;Chu;China J. Chin. Mater. Medica,2023

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