Associations of the Single Bovine Embryo Growth Media Metabolome with Successful Pregnancy

Author:

Tsopp Elina1ORCID,Kilk Kalle2ORCID,Taalberg Egon2,Pärn Pille1,Viljaste-Seera Anni1,Kavak Ants3,Jaakma Ülle1

Affiliation:

1. Chair of Animal Breeding and Biotechnology, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia

2. Department of Biochemistry, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, Estonia

3. Chair of Clinical Veterinary Medicine, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia

Abstract

This study investigated whether metabolomic fingerprints of bovine embryo growth media improve the prediction of successful embryo implantation. In this prospective cohort study, the metabolome from in vitro-produced day 7 blastocysts with successful implantation (n = 11), blastocysts with failed implantation (n = 10), and plain culture media without embryos (n = 5) were included. Samples were analyzed using an AbsoluteIDQ® p180 Targeted Metabolomics Kit with LC-MS/MS, and a total of 189 metabolites were analyzed from each sample. Blastocysts that resulted in successful embryo implantation had significantly higher levels of methionine sulfoxide (p < 0.001), DOPA (p < 0.05), spermidine (p < 0.001), acetylcarnitine-to-free-carnitine ratio (p < 0.05), C2 + C3-to-free-carnitine ratio (p < 0.05), and lower levels of threonine (nep < 0.001) and phosphatidylcholine PC ae C30:0 (p < 0.001) compared to control media. However, when compared to embryos that failed to implant, only DOPA, spermidine, C2/C0, (C2 + C3)/C0, and PC ae C30:0 levels differentiated significantly. In summary, our study identifies a panel of differential metabolites in the culture media of bovine blastocysts that could act as potential biomarkers for the selection of viable blastocysts before embryo transfer.

Funder

European Union

European Regional Development Fund

Estonian Research Council

Publisher

MDPI AG

Subject

Molecular Biology,Biochemistry,Endocrinology, Diabetes and Metabolism

Reference52 articles.

1. New Approaches to Diagnose and Target Reproductive Failure in Cattle;Pohler;Anim. Reprod.,2020

2. Embryo Transfer in Cattle Production and Its Principle and Applications;Mebratu;Int. J. Pharm. Biomed. Res.,2020

3. Embryo Transfer as an Option to Improve Fertility in Repeat Breeder Dairy Cows;Nowicki;J. Vet. Res.,2021

4. 2021 Statistics of Embryo Production and Transfer in Domestic Farm Animals;Viana;Embryo Technol. Newsl.,2022

5. The Incompletely Fulfilled Promise of Embryo Transfer in Cattle-Why Aren’t Pregnancy Rates Greater and What Can We Do about It?;Hansen;J. Anim. Sci.,2020

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3