New Insight into the Genus Cladocroce (Porifera, Demospongiae) Based on Morphological and Molecular Data, with the Description of Two New Species

Author:

Bertolino Marco1,Cerrano Carlo2ORCID,Bavestrello Giorgio1ORCID,Thung Do Cong3,Núñez-Pons Laura45ORCID,Rispo Francesca1ORCID,Efremova Jana45,Mazzella Valerio45ORCID,Makapedua Daisy Monica6,Calcinai Barbara2ORCID

Affiliation:

1. Department of Earth, Environmental and Life Sciences (DISTAV), University of Genova, Corso Europa 26, 16132 Genova, Italy

2. Department of Life and Environmental Sciences (DISVA), Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy

3. Institute of Marine Environment and Resources (IMER), 246 Danang Street, Haiphong City 180000, Vietnam

4. Department of Integrative Marine Ecology (EMI), Stazione Zoologica Anton Dohrn (SZN), Villa Comunale, 80121 Napoli, Italy

5. National Biodiversity Future Center (NBFC), 90133 Palermo, Italy

6. Department of Fishery Product Processing, Faculty of Fisheries and Marine Science, University of Sam Ratulangi, Jl. Kampus Unsrat, Manado 95115, Indonesia

Abstract

During scientific expeditions in Indonesia and Vietnam, several sponge specimens belonging to the genus Cladocroce were collected. The integration of morphological and molecular analyses, incorporating species delimitation models (ABGD, ASAP, and bPTP) and phylogenetic approaches using three molecular markers (COI, 28S, and 18S–ITS1–5.8S–ITS2–28S), allowed us to discriminate three congeneric species. Two of these species (C. burapha and C. pansinii sp. nov.) were supported by morphological and molecular data, whereas a third species (C. lamellata sp. nov.) was delimited by morphological data only. We formally describe two new species, C. pansinii sp. nov. and C. lamellata sp. nov. C. aculeata is a newly recorded species for Indonesia and the first documented finding after the original description. The re-examination of the type material of C. burapha, and indirectly the molecular approach, allowed us to confirm that C. burapha lives in sympatry with C. pansinii sp. nov. in Vietnam and with C. lamellata in Indonesia. Thanks to these findings, we relocated the paratype of C. burapha to the new species described here, i.e., C. pansinii sp. nov.

Publisher

MDPI AG

Subject

Ocean Engineering,Water Science and Technology,Civil and Structural Engineering

Reference46 articles.

1. First record of Cladocroce (Porifera: Haplosclerida: Chalinidae) from the eastern pacific ocean with the description of Cladocroce reina sp. nov;Carballo;Zootaxa,2010

2. De Weerdt, W.H. (2002). Systema Porifera, Springer.

3. De Voogd, N.J., Alvarez, B., Boury-Esnault, N., Carballo, J.L., Cárdenas, P., Díaz, M.-C., Dohrmann, M., Downey, R., Hajdu, E., and Hooper, J.N.A. (2022, October 12). World Porifera Database. Available online: https://www.marinespecies.org/porifera.

4. Cladocroce caelum sp nov from the Brazilian coast; First record of the genus in the South Atlantic;Santos;Zootaxa,2014

5. Mangrove sponges from Bangka Island (North Sulawesi, Indonesia) with the description of a new species;Calcinai;J. Mar. Biol. Assoc.,2017

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